NISCAIR Online Periodicals Repository

Research Journals >
Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.67 [2008] >
JSIR Vol.67(01) [January 2008] >

Title: Partial characterization and optimization of extracellular thermostable Ca²⁺ inhibited α-amylase production by Streptomyces erumpens MTCC 7317
Authors: Kar, S
Ray, R C
Keywords: Actinomycete
Streptomyces erumpens MTCC 7317
Issue Date: Jan-2008
Publisher: CSIR
Abstract: Culture parameters for optimum production of extracellular a-amylase by moderately thermostable Streptomyces erumpens MTCC 7317 isolated from a brick-kiln soil were found to be pH (6.0), temperature (50°C) and incubation period (36 h). Enzyme production was more in soluble starch (1%), beef extract (1%) and glycerol (0.02%) as compared to other carbon (1%: wheat flour, cassava starch, potato starch, cassava flour, etc.), nitrogen (1%: yeast extract, peptone, casein, ammonium chloride, etc.) and surfactant [0.02%: Cween (Tween) 20, Cween 40, Cween 60, Cween 80, sodium lauryl sulphate (SLS) and triton x 100] sources tested, respectively. Glycerol concentration (0.02 %) was most stimulatory in enzyme production. Addition of Ca²⁺ (even at 10 mM) inhibited a-amylase production. S. erumpens cells immobilized on sodium alginate beads produced more (9.0-23.4%) amylase as compared with free cells. Partially purified enzyme (by ammonium sulphate precipitation) had a molecular mass of 54,500 Da in native SDS-PAGE.
Page(s): 58-64
Source:JSIR Vol.67(01) [January 2008]

Files in This Item:

File Description SizeFormat
JSIR 67(1) (2008) 58-64.pdf345.67 kBAdobe PDFView/Open
 Current Page Visits: 949 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 164898 since 01-Sep-2015  Last updated on 27-Jun-2016Webmaster: