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JSIR Vol.67 [2008] >
JSIR Vol.67(01) [January 2008] >
| Title: | Partial characterization and optimization of extracellular thermostable Ca²⁺ inhibited α-amylase production by Streptomyces erumpens MTCC 7317 |
| Authors: | Kar, S
Ray, R C |
| Keywords: | Actinomycete
α-Amylase
Streptomyces erumpens MTCC 7317
Thermostable |
| Issue Date: | Jan-2008 |
| Publisher: | CSIR |
| Abstract: | Culture parameters for optimum production of extracellular a-amylase by moderately thermostable Streptomyces
erumpens MTCC 7317 isolated from a brick-kiln soil were found to be pH (6.0), temperature (50°C) and incubation period
(36 h). Enzyme production was more in soluble starch (1%), beef extract (1%) and glycerol (0.02%) as compared to other
carbon (1%: wheat flour, cassava starch, potato starch, cassava flour, etc.), nitrogen (1%: yeast extract, peptone, casein,
ammonium chloride, etc.) and surfactant [0.02%: Cween (Tween) 20, Cween 40, Cween 60, Cween 80, sodium lauryl
sulphate (SLS) and triton x 100] sources tested, respectively. Glycerol concentration (0.02 %) was most stimulatory in
enzyme production. Addition of Ca²⁺ (even at 10 mM) inhibited a-amylase production. S. erumpens cells immobilized on
sodium alginate beads produced more (9.0-23.4%) amylase as compared with free cells. Partially purified enzyme (by
ammonium sulphate precipitation) had a molecular mass of 54,500 Da in native SDS-PAGE. |
| Page(s): | 58-64 |
| Source: | JSIR Vol.67(01) [January 2008]
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