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Title: Online HPLC-DPPH method for antioxidant activity of <i style="">Picrorhiza kurroa</i> Royle ex Benth. and characterization of kutkoside by Ultra-Performance LC-electrospray ionization quadrupole time-of-flight mass spectrometry
Authors: Bhandari, Pamita
Kumar, Neeraj
Singh, Bikram
Ahuja, Paramvir S
Keywords: Antioxidant activity
<i style="">Picrorhiza kurroa</i>
Ultra-performance liquid chromatography
Issue Date: Mar-2010
Publisher: CSIR
Abstract: <i style="">Picrorhiza kurroa</i> Royle ex Benth., is widely used in the Indian systems of medicine for the treatment of various liver ailments. Since, the role of oxi­dative stress in the pathogenesis of liver injury has become generally recognized, in present study the free radical scavenging effect of <i style="">P. kurroa</i> was assessed by on-line HPLC-DPPH and colorimetric DPPH methods. The comparative study on antioxidant activity of <i style="">P. kurroa</i> extracts by both methods revealed that colorimetric method showed very less free radical scavenging effect while HPLC-DPPH method showed high activity. Further, the kutkoside, an important ingredient of a potent hepatoprotective formulation “kutkin/ picroliv” was investigated for its chemical composition by ultra-performance liquid chromatography coupled with diode array detection/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-DAD/ESI-QTOF-MS). Kutkoside was considered to be a single compound and reported as picroside-II or kutkoside, however, present investigation illustrated that kutkoside is a mixture of iridoid glycosides namely, picroside II, picroside IV and 6-ferulloylcatalpol.
Description: 323-328
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.48(03) [March 2010]

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