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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(03) [March 2010] >
| Title: |  -Amyrin from Ardisia elliptica is more potent than aspirin in inhibiting collagen-induced platelet aggregation |
| Authors: | Ching, Jianhong
Chua, Tung-Kian
Chin, Lee-Cheng
Lau, Aik-Jiang
Pang, Yun-Keng
Jaya, Johannes Murti
Tan, Chay-Hoon
Koh, Hwee-Ling |
| Keywords: | -Amyrin
Ardisia elliptica
Collagen
Platelet aggregation |
| Issue Date: | Mar-2010 |
| Publisher: | CSIR |
| Abstract: | Ardisia elliptica Thunberg
(Myrsinaceae) is a medicinal plant traditionally used for alleviating chest
pains, treatment of fever, diarrhoea, liver poisoning and parturition
complications. The objectives of
the study were to investigate the effect of A. elliptica on
collagen induced platelet aggregation and to isolate and purify potential
antiplatelet components. Fresh
A.
elliptica leaves were extracted using
methanol (70% v/v) by Soxhlet extraction and the extract was analysed for its
inhibition of collagen-induced platelet aggregation. Inhibition of platelet
aggregation was assessed by incubating the extracts with rabbit blood and
collagen in a whole blood aggregometer and measuring the impedance. The leaf
extract was found to inhibit platelet aggregation with an IC50 value of 167
 g/ml. Using bioassay guided fractionation, -amyrin was isolated and purified. The IC50
value of -amyrin was found to be 4.5
g/ml (10.5 M) while that of aspirin was found to be
11 g/ml (62.7 M), indicating that -amyrin was six times as active as aspirin in inhibiting platelet
aggregation. This paper is the first report that -amyrin isolated from A.
elliptica is more potent than aspirin in inhibiting collagen-induced
platelet aggregation. In conclusion, A. elliptica leaves were found to inhibit collagen-induced platelet aggregation
and one of the bioactive components responsible for the observed effect was
determined to be -amyrin. |
| Page(s): | 275-279 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(03) [March 2010]
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