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JSIR Vol.69(03) [March 2010] >


Title: Enzymatic hydrolysis of chemically pretreated rice straw by two indigenous fungal strains: a comparative study
Authors: Singh, Anita
Singh, Namita
Bishnoi, Narsi R
Keywords: Cellulase
Lignocellulose
Pretreatment
Aspergillus heteromorphus
A. niger
Issue Date: Mar-2010
Publisher: CSIR
Abstract:  This study presents pretreatment of rice straw using ethylene diamine, (EDA 28%), hydrochloric acid (HCl 5%) and sodium hydroxide (NaOH 2%) and on-site enzyme production from two Aspergillus species. Pretreated rice straw was enzymatically hydrolyzed with crude cellulase from A. niger and A. heteromorphus. Enzymatic hydrolysis conditions (pH and temperature) were optimized by RSM model. Maximum activity was found by A. niger (FPase, 7.4 IU/g; CMCase, 97 IU/g) and A. heteromorphus (FPase, 9.5 IU/g; CMCase 125 IU/g) in 72 h with EDA pretreated rice straw followed by NaOH and HCl pretreatment.
Page(s): 232-237
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.69(03) [March 2010]

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