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Title: Enzymatic hydrolysis of chemically pretreated rice straw by two indigenous fungal strains: a comparative study
Authors: Singh, Anita
Singh, Namita
Bishnoi, Narsi R
Keywords: Cellulase
<i>Aspergillus heteromorphus</i>
<i>A. niger</i>
Issue Date: Mar-2010
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> This study presents pretreatment of rice straw using ethylene diamine, (EDA 28%), hydrochloric acid (HCl 5%) and sodium hydroxide (NaOH 2%) and on-site enzyme production from two <i>Aspergillus </i>species. Pretreated rice straw was enzymatically hydrolyzed with crude cellulase from <i>A. niger </i>and <i>A. heteromorphus</i>. Enzymatic hydrolysis conditions (pH and temperature) were optimized by RSM model. Maximum activity was found by <i>A. niger </i>(FPase, 7.4 IU/g; CMCase, 97 IU/g) and <i>A. heteromorphus </i>(FPase, 9.5 IU/g; CMCase 125 IU/g) in 72 h with EDA pretreated rice straw followed by NaOH and HCl pretreatment. </smarttagtype></smarttagtype>
Description: 232-237
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.69(03) [March 2010]

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