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Title: Micropropagation of Ceropegia hirsute Wt. & Arn.—A starchy tuberous asclepid
Authors: Nikam, T D
Savant, R S
Pagare, R S
Keywords: BAP
Ceropegia hirsute
micropropagation
nodal explants
Issue Date: Jan-2008
Publisher: CSIR
Abstract: A protocol is described for micropropagation of the starchy, tuberous, herbaceous twinner, Ceropegia hirsute Wt. & Arm. by in vitro culture of nodal segments. Of the cytokinins (BAP and Kn) and auxins (IAA, NAA, 2,4-D) evaluated as supplements individually and in combinations to Murashige and Skoog (MS) medium, BAP (7.5 µM) was the most effective in inducing axillary multiple shoots (5.7±0.7 shoots/culture). Root were induced to in vitro derived shoots on half-strength MS medium supplemented with IAA (2 µM) and sucrose (5%). in vitro plants were acclimatized and were successfully established in the soil.
Page(s): 129-132
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source: IJBT Vol.07(1) [January 2008]

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