Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/7331
Title: Micropropagation of <i>Ceropegia hirsute </i>Wt. & Arn.—A starchy tuberous asclepid
Authors: Nikam, T D
Savant, R S
Pagare, R S
Keywords: BAP
<i>Ceropegia hirsute</i>
micropropagation
nodal explants
Issue Date: Jan-2008
Publisher: CSIR
Abstract: A protocol is described for micropropagation of the starchy, tuberous, herbaceous twinner, <i>Ceropegia</i> <i>hirsute</i> Wt. & Arm. by <i>in vitro</i> culture of nodal segments. Of the cytokinins (BAP and Kn) and auxins (IAA, NAA, 2,4-D) evaluated as supplements individually and in combinations to Murashige and Skoog (MS) medium, BAP (7.5 µ<i style="">M</i>) was the most effective in inducing axillary multiple shoots (5.7±0.7 shoots/culture). Root were induced to <i style="">in vitro </i>derived shoots on half-strength MS medium supplemented with IAA (2 µ<i style="">M</i>) and sucrose (5%). <i>in vitro</i> plants were acclimatized and were successfully established in the soil.
Description: 129-132
URI: http://hdl.handle.net/123456789/7331
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections: IJBT Vol.07(1) [January 2008]

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