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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.07 [2008] >
IJBT Vol.07(1) [January 2008] >
| Title: | Optical resolution of (R, S)-ibuprofen in organic solvent by porcine pancreatic lipase catalyzed enantioselective esterification |
| Authors: | Ramachandran, Ganesh
Ananthanarayan, Laxmi |
| Keywords: | Enantioselectivity
esterification
ibuprofen
NSAIDS
optical resolution
porcine pancreatic lipase
racemates |
| Issue Date: | Jan-2008 |
| Publisher: | CSIR |
| Abstract: | Porcine pancreatic lipase (PPL), a comparatively inexpensive enzyme, was used in the optical resolution of (R, S)-ibuprofen in methanol through enantioselective esterification. The different reaction parameters during the esterification of (R, S)-ibuprofen using PPL was studied with respect to temperature, time, enzyme concentration, substrate concentration and water content. The percentage conversion initially was 13%, which increased to 33% after optimization of the different reaction conditions. Surfactant coating of PPL resulted in a 2.5-fold increase in the hydrolytic activity of PPL. Surfactant coated PPL, when used in the esterification reaction slightly increased the percentage conversion from 21 to 22.5%. In all the reactions, PPL showed preference for catalyzing the esterification of S-(+)-ibuprofen. |
| Page(s): | 94-98 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.07(1) [January 2008]
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