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|Title:||Cloning and characterization of the gene encoding HMG CoA reductase from black night shade (Solanum nigrum L.)|
Beena, P S
|Keywords:||HMGR;in-silico analysis;mevalonate pathway;polymerase chain reaction|
|Abstract:||The first committed step in the pathway for biosynthesis of isoprenoids in plants is catalyzed by 3-hydroxy 3- methylglutaryl CoA reductase (HMGR; EC:220.127.116.11). Here we report for the first time, the cloning of a partial genomic DNA sequence encoding HMGR from a medicinal herb, Solanum nigrum L. This plant is associated with resistance against potato cyst nematode and the late blight pathogen, Phytophthora infestans. The clone Snhmgr (GenBank Acc.No. DQ 229901) had 582 base pairs (bp) with a 462 bp open reading frame (ORF), encoding 142 amino acid polypeptide. The in-silico analysis of Snhmgr sequence revealed about 85% identity with hmgr genes in other solanaceous plants. Phylogenetic analysis indicated that Snhmgr was more identical with tomato hmgr on evolutionary basis and belonged to the solanaceous cluster, including hmgr genes from tomato, potato, tobacco and capsicum. Functional analysis of conserved domains of Snhmgr showed CoA reductase, NAD binding and substrate binding activities.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:|| IJBT Vol.07(1) [January 2008]|
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