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|Title:||Cloning and characterization of the gene encoding HMG CoA reductase from black night shade (<i>Solanum nigrum </i>L.)<b style=""></b>|
Beena, P S
polymerase chain reaction
|Abstract:||The first committed step in the pathway for biosynthesis of isoprenoids in plants is catalyzed by 3-hydroxy 3- methylglutaryl CoA reductase (HMGR; EC:188.8.131.52). Here we report for the first time, the cloning of a partial genomic DNA sequence encoding HMGR from a medicinal herb, <i>Solanum nigrum </i>L. This plant is associated with resistance against potato cyst nematode and the late blight pathogen, <i>Phytophthora infestans</i>. The clone <i>Snhmgr </i>(GenBank Acc.No. DQ 229901) had 582 base pairs (bp) with a 462 bp open reading frame (ORF), encoding 142 amino acid polypeptide. The <i>in-silico</i> analysis of <i>Snhmgr </i>sequence revealed about 85% identity with <i>hmgr</i> genes in other solanaceous plants. Phylogenetic analysis indicated that <i>Snhmgr </i>was more identical with tomato <i>hmgr</i> on evolutionary basis and belonged to the solanaceous cluster, including <i>hmgr</i> genes from tomato, potato, tobacco and capsicum. Functional analysis of conserved domains of <i>Snhmgr</i> showed CoA reductase, NAD binding and substrate binding activities.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:|| IJBT Vol.07(1) [January 2008]|
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