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Title: Regeneration of multiple shoots from the callus cultures of Macrotyloma uniflorum (Lam.) Verdc.
Authors: Tejavathi, D H
Devaraj, V R
Murthy, Savitha M
Anitha, P
Nijagunaiah, R
Keywords: Callus culture
histology
horse gram
Macrotyloma uniflorum
organogenesis
Issue Date: Jan-2010
Publisher: CSIR
Abstract: An effective protocol for the regeneration of multiple shoots from the callus, derived from shoot tip and cotyledonary node, was developed for the first time for Macrotyloma uniflorum (Lam.) Verdc. Shoot apices and cotyledonary nodes from 9-d-old aseptically grown seedlings were inoculated onto MS, L2 and MMS media supplemented with various growth regulators. Proliferation of about 17 shoots was obtained on AS supplemented MS medium after 16 d of culture, while about 14 and 20 shoots regenerated from the callus on MMS1+NAA (1.86 μM)+BAP (0.45 μM) and L2+IBA (0.45 μM)+BAP (0.11 μM), respectively after 30 d of culture. Regenerated shoots were rooted on MS+IAA (0.11 µM)+Kn (0.21 µM) with upto 6 roots per shoot. Rooted shoots were sequentially acclimatized by transferring them to sterile distilled water and tap water before planting in various pot mixtures. A maximum of 60% survival rate was noticed on a mixture of soil:sand:manure (1:1:1).
Page(s): 101-105
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(1) [January 2010]

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