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Title: Regeneration of multiple shoots from the callus cultures of <i style="">Macrotyloma uniflorum </i>(Lam.) Verdc.
Authors: Tejavathi, D H
Devaraj, V R
Murthy, Savitha M
Anitha, P
Nijagunaiah, R
Keywords: Callus culture
horse gram
<i style="">Macrotyloma uniflorum</i>
Issue Date: Jan-2010
Publisher: CSIR
Abstract: An effective protocol for the regeneration of multiple shoots from the callus, derived from shoot tip and cotyledonary node, was developed for the first time for <i style="">Macrotyloma uniflorum </i>(Lam.) Verdc. Shoot apices and cotyledonary nodes from 9-d-old aseptically grown seedlings were inoculated onto MS, L<sub>2</sub> and MMS media supplemented with various growth regulators. Proliferation of about 17 shoots was obtained on AS supplemented MS medium after 16 d of culture, while about 14 and 20 shoots regenerated from the callus on MMS<sub>1</sub>+NAA (1.86 μ<i>M</i>)+BAP (0.45 μ<i>M</i>) and L<sub>2</sub>+IBA (0.45 μ<i>M</i>)+BAP (0.11 μ<i>M</i>), respectively after 30 d of culture. Regenerated shoots were rooted on MS+IAA (0.11 µ<i>M</i>)+Kn (0.21 µ<i>M</i>) with upto 6 roots per shoot. Rooted shoots were sequentially acclimatized by transferring them to sterile distilled water and tap water before planting in various pot mixtures. A maximum of 60% survival rate was noticed on a mixture of soil:sand:manure (1:1:1).
Description: 101-105
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(1) [January 2010]

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