Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/7128
Title: Differentiation of petroleum hydrocarbon-degrading <i style="">Pseudomonas </i>spp. based on lectin binding of cell extracts in an agglutination assay
Authors: Tanti, Bhaben
Buragohain, Alak Kumar
Keywords: Agglutination assay
carbohydrate moieties
lectin typing
<i style="">Pseudomonas </i>spp.
Issue Date: Jan-2010
Publisher: CSIR
Abstract: Plant and animal lectins with various carbohydrate specificities were used to type 43 petroleum hydrocarbon-degrading <i>Pseudomonas</i> isolates and the type strain <i>P. aeruginosa</i> MTCC1034 in a microtiter plate assay. Initially, a panel of nine lectins with their known specificity was used. For optimal typing, pretreatment by washing bacteria with a low <i>p</i>H buffer to allow protein release, followed by proteolytic degradation to eliminate autoagglutination, was used. Lectin types of treated samples were stable and reproducible. No strain proved to be untypeable by this system. The species were clustered into 10 different lectin types on the basis of their lectin binding with specific bacterial cell surface carbohydrate moieties. The result of lectin typing reveals considerable intraspecific variations within the species<i style="">.</i>
Description: 74-79
URI: http://hdl.handle.net/123456789/7128
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(1) [January 2010]

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