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|Title:||Differentiation of petroleum hydrocarbon-degrading Pseudomonas spp. based on lectin binding of cell extracts in an agglutination assay|
Buragohain, Alak Kumar
|Keywords:||Agglutination assay;carbohydrate moieties;lectin typing;Pseudomonas spp.|
|Abstract:||Plant and animal lectins with various carbohydrate specificities were used to type 43 petroleum hydrocarbon-degrading Pseudomonas isolates and the type strain P. aeruginosa MTCC1034 in a microtiter plate assay. Initially, a panel of nine lectins with their known specificity was used. For optimal typing, pretreatment by washing bacteria with a low pH buffer to allow protein release, followed by proteolytic degradation to eliminate autoagglutination, was used. Lectin types of treated samples were stable and reproducible. No strain proved to be untypeable by this system. The species were clustered into 10 different lectin types on the basis of their lectin binding with specific bacterial cell surface carbohydrate moieties. The result of lectin typing reveals considerable intraspecific variations within the species.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.09(1) [January 2010]|
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