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Title: Investigation on relative genome sizes and ploidy levels of Darjeeling-Himalayan <i style="">Rhododendron </i>species<i style=""> </i>using flow cytometer
Authors: De, Kalyan Kumar
Saha, Aniruddha
Tamang, Ranju
Sharma, Bilok
Keywords: Flow cytometer
genome size
<i style="">Rhododendron</i>
Issue Date: Jan-2010
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="metricconverter"> The relative 2C genome size (in pg), ploidy level (X) and total number of base pair (in Mbp) of ten threatened, rare and endangered Indian <i style="">Rhododendron</i> species of Darjeeling Hills (eastern Himalaya) were determined by using flow cytometer. Out of the 10 species, 9 were diploid and their genome sizes (2C) ranged from 1.30 to 1.51 pg. But in one species, i.e., <i style="">R. niveum</i>, the value of genome size was significantly high, i.e., 4.27 pg, appeared to be a natural hexaploid (6X). The cytometric genomic data indicating the hexaploid nature of <i style="">R. niveum</i> can be correlated with some phenotypic characters, specifically the size of leaf, size of flower and number of flower in truss. The reported phenotypic character of diploid species of <i style="">R. niveum</i> reveals that leaf size varies from 9-12 cm in length and 3.5-5.5 cm in breadth, individual flower size varies from 2-2.5 cm in length and 2-2.5 cm in diameter and number of flower per truss is 18-23 in diploid species. However, the findings on the same phenotypic characters of hexaploid R<i style="">. niveum</i> in the present study showed some differences from the diploid one. The leaf size of hexaploid <i style="">R. niveum </i>varies from 15-20 cm in length and 5-7 cm in breadth, individual flower size varies from 3.5-4 cm in length and 3.5 cm in diameter and number of flower per truss is 25-30. Therefore, these differences in floral traits and leaf size may be direct result of polyploidization itself. Thus, the morphological traits and the genomic information of <i style="">Rhododendron</i> species may serve as a valuable database for Indian <i style="">Rhododendron </i>breeders. </smarttagtype>
Description: 64-68
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(1) [January 2010]

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