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Title: Investigation on relative genome sizes and ploidy levels of Darjeeling-Himalayan Rhododendron species using flow cytometer
Authors: De, Kalyan Kumar
Saha, Aniruddha
Tamang, Ranju
Sharma, Bilok
Keywords: Flow cytometer;genome size;Rhododendron
Issue Date: Jan-2010
Publisher: CSIR
Abstract:  The relative 2C genome size (in pg), ploidy level (X) and total number of base pair (in Mbp) of ten threatened, rare and endangered Indian Rhododendron species of Darjeeling Hills (eastern Himalaya) were determined by using flow cytometer. Out of the 10 species, 9 were diploid and their genome sizes (2C) ranged from 1.30 to 1.51 pg. But in one species, i.e., R. niveum, the value of genome size was significantly high, i.e., 4.27 pg, appeared to be a natural hexaploid (6X). The cytometric genomic data indicating the hexaploid nature of R. niveum can be correlated with some phenotypic characters, specifically the size of leaf, size of flower and number of flower in truss. The reported phenotypic character of diploid species of R. niveum reveals that leaf size varies from 9-12 cm in length and 3.5-5.5 cm in breadth, individual flower size varies from 2-2.5 cm in length and 2-2.5 cm in diameter and number of flower per truss is 18-23 in diploid species. However, the findings on the same phenotypic characters of hexaploid R. niveum in the present study showed some differences from the diploid one. The leaf size of hexaploid R. niveum varies from 15-20 cm in length and 5-7 cm in breadth, individual flower size varies from 3.5-4 cm in length and 3.5 cm in diameter and number of flower per truss is 25-30. Therefore, these differences in floral traits and leaf size may be direct result of polyploidization itself. Thus, the morphological traits and the genomic information of Rhododendron species may serve as a valuable database for Indian Rhododendron breeders.
Page(s): 64-68
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(1) [January 2010]

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