NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(1) [January 2010] >


Title: Identification of RAPD markers linked to plant type gene in pigeonpea
Authors: Dhanasekar, P
Dhumal, K N
Reddy, K S
Keywords: Cajanus cajan
compact-dwarf
pigeonpea
plant type
marker
RAPD
Issue Date: Jan-2010
Publisher: CSIR
Abstract:  Pigeonpea is the second most important pulse crop of India accounting for almost 90% of the World’s pigeonpea area and production. Altering the plant types could improve the economic yield and its adaptability as well. Molecular markers can identify and tag plant type genes without much environmental interference. In the present investigation of identifying and tagging RAPD markers linked with the plant type trait, the plant material consisted of parents, F1 and a F2 population obtained by crossing genotypes TT44-4 with open-tall and TDI2004-1 with compact-dwarf plant types. RAPD analysis was performed on 84 genotyped F2 plants following a bulked segregant analysis (BSA) approach. In the parental screening with 200 random decamer primers, only eight showed polymorphism, of which only two could be linked to the plant type gene. Two markers (OPF04700 and OPA091375) were identified that were present in the open-tall plants, while absent in compact-dwarf plants. Linkage analysis showed that the markers OPF04700 and OPA091375 were located 8.4±0.03 cM and 9.6±0.032 cM, respectively away from the plant type gene locus. The markers were validated in 15 genotypes with open-tall plant type. The utilization of the marker and the dwarfing gene is discussed.
Page(s): 58-63
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(1) [January 2010]

Files in This Item:

File Description SizeFormat
IJBT 9(1) 58-63.pdf1.23 MBAdobe PDFView/Open
 Current Page Visits: 1016 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 559458 since 06-Feb-2009  Last updated on 30-Jul-2014Webmaster: nopr@niscair.res.in