Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/7122
Title: Strain differentiation and characterization of canine parvovirus by PCR and RE mapping
Authors: Nandi, S
Anbuzhagan, R
Kumar, Manoj
Keywords: Canine parvovirus
haemorrhagic enteritis
MDCK
PCR
RE mapping
Issue Date: Jan-2010
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> Canine parvovirus 2 (CPV2) is a highly contagious and fatal disease of dogs, causing acute hemorrhagic enteritis and myocarditis. In this study, different mutant of CPV2 prevalent in India have been isolated in Madin-Darby canine kidney (MDCK) cells and characterized by polymerase chain reaction (PCR) and restriction endonuclease (RE) mapping. The faecal samples from gastroenteritic dog suspected for CPV2 infection were collected in a suitable medium, processed and inoculated in MDCK cells. The viral DNA from faecal samples was extracted using phenol-chloroform method. PCR were carried out with two different primer pairs, pCPV-2ab and pCPV-2b, to distinguish the strain prevalent in field condition. The primer pCPV-2ab recognized both the variant CPV-2a and CPV-2b, whereas the primer pCPV-2b recognized only the variant CPV-2b, enabling the differentiation of CPV-2a variant from CPV-2b in field isolates. The different PCR products were further analyzed by using RE mapping. </smarttagtype></smarttagtype>
Description: 38-42
URI: http://hdl.handle.net/123456789/7122
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(1) [January 2010]

Files in This Item:
File Description SizeFormat 
IJBT 9(1) 38-42.pdf826.76 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.