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|Title:||Strain differentiation and characterization of canine parvovirus by PCR and RE mapping|
|Abstract:||<smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> Canine parvovirus 2 (CPV2) is a highly contagious and fatal disease of dogs, causing acute hemorrhagic enteritis and myocarditis. In this study, different mutant of CPV2 prevalent in India have been isolated in Madin-Darby canine kidney (MDCK) cells and characterized by polymerase chain reaction (PCR) and restriction endonuclease (RE) mapping. The faecal samples from gastroenteritic dog suspected for CPV2 infection were collected in a suitable medium, processed and inoculated in MDCK cells. The viral DNA from faecal samples was extracted using phenol-chloroform method. PCR were carried out with two different primer pairs, pCPV-2ab and pCPV-2b, to distinguish the strain prevalent in field condition. The primer pCPV-2ab recognized both the variant CPV-2a and CPV-2b, whereas the primer pCPV-2b recognized only the variant CPV-2b, enabling the differentiation of CPV-2a variant from CPV-2b in field isolates. The different PCR products were further analyzed by using RE mapping. </smarttagtype></smarttagtype>|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.09(1) [January 2010]|
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