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IJBT Vol.09(1) [January 2010] >


Title: RAPD marker system in insect study: A review
Authors: Jain, Subodh Kumar
Neekhra, Bharat
Pandey, Divya
Jain, Kalpana
Keywords: Genetic variation
insects
molecular markers
PCR
RAPD
Issue Date: Jan-2010
Publisher: CSIR
Abstract: Insects represent a major life form on earth. So far, nearly 0.9 million insect species are discovered, comprising 75% of all the recorded animal species. Some of the insect species are easy to identify and categorize, while for others, it is difficult because of their small size and morphological similarity. Moreover, it is further difficult to identify morphological variation due to environmental factors by available traditional methods. To overcome these problems, the advanced molecular techniques, viz., PCR (Polymerase Chain Reaction), RFLP (Restriction Fragment Length Polymorphism), RAPD (Random Amplified Polymorphic DNA), and AFLP (Arbitrary Fragment Length Polymorphism) have been a great help. RAPD markers have been used in gene mapping to characterize cultivars and species genetically, infer phylogeny and biogeography of insect population and understand modes of evolution and evolutionary trajectories. Thus, RAPD markers have become the most common yardsticks for measuring genetic differences between individuals, within and between related species or population. The unprecedented advancements in modern molecular biology, particularly in those of DNA marker technology, have created a wealth of technical know-how that finds useful application in molecular ecology research in insects.
Page(s): 7-12
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(1) [January 2010]

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