NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.13 [2006] >
IJCT Vol.13(3) [May 2006] >

Title: Stability-indicating HPLC determination of tolterodine tartrate in pharmaceutical dosage form
Authors: Saxena, Vinay
Zaheer, Zahid
Farooqui, Mazhar
Keywords: Tolterodine tartrate
Stability indicating
Issue Date: May-2006
Publisher: CSIR
IPC CodeB01D15/08
Abstract: A simple, selective, precise and stability-indicating high-performance liquid chromatographic (HPLC) method of analysis tolterodine tartrate in pharmaceutical dosage form was developed and validated. The chromatographic conditions comprised a reversed-phase C18 column (250 4.6 mm), 5 µ with a mobile phase consisting of a mixture of buffer solution (2.88 g ammonium dihydrogen orthophosphate in 1 L of water) and methanol in the ratio of 40 : 60. Triethylamine (5 mL /L) was added to it and pH of mobile phase was adjusted to 7.0± 0.1 with orthophosphoric acid at a flow rate of 1.5 mL/min. Detection was carried out at 220 nm. The retention time of tolterodine was 6.49 min. The linear regression analysis data for the calibration plots showed good linear relationship with coefficient of regression value, r2 = 0.99 in the concentration range 200.60-601.80 µg per mL. The value of correlation coefficient, slope and intercept were 1.0, 20.87 and –6.87, respectively. The method was validated for precision, recovery, ruggedness and robustness. The drug undergoes degradation under acidic, basic, photochemical and thermal conditions. All the peaks of degraded product were resolved from the active pharmaceutical ingredient with significantly different retention time. The samples degraded with hydrogen peroxide showed no additional peak. This indicates that the drug is susceptible to acid-base hydrolysis, photochemical and thermal degradation. Statistical analysis proves that the method is reproducible and selective for the estimation of said drug. As the method could effectively separate the drug from its degradation product, it can be employed as a stability-indicating one.
Page(s): 242-246
ISSN: 0975-0991 (Online); 0971-457X (Print)
Source:IJCT Vol.13(3) [May 2006]

Files in This Item:

File Description SizeFormat
IJCT 13(3) 242-246.pdf63.55 kBAdobe PDFView/Open
 Current Page Visits: 1538 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171564 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: