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|Title:||Production of bioemulsifier by Acinetobacter species isolated from healthy human skin|
|Keywords:||Acinetobacter;Bioemulsifier;Cleaning property;Emulsion;Human skin;Proteoglycan|
|Abstract:||Six Acinetobacter sp. isolated from healthy human skin were checked for the production of bioemulsifier. Optimization studies indicated that Luria Bertani broth pH 7 supplemented with calcium chloride (1%) was the optimum medium. Temperature at 37°C was optimum and inducer oils in the medium did not enhance bioemulsifier production. Partial purification of bioemulsifier and chemical analysis revealed that it is a proteoglycan with protein (53%), polysaccharide (43%) and lipid (2%). Maximum emulsification activity obtained was 400 EU/ml. Thin layer chromatography revealed the presence of mannose and rhamnose sugar and oleic and palmitic acids as parts of lipids. The yield obtained was 1.9 g / l. Reconstitution studies revealed that the protein and polysaccharide fractions together display 94.55% of emulsification activity. It was also noted that the bioemulsifier was stable for 72 hr at 37°C and displayed good cleaning property towards different oils. The partially purified bioemulsifier formed stable oil-in-water emulsions with plant oils.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.48(01) [January 2010]|
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