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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(01) [January 2010] >
| Title: | Production of bioemulsifier by Acinetobacter species isolated from healthy human skin |
| Authors: | Jagtap, Shweta
Yavankar, Supriya
Pardesi, Karishma
Chopade, Balu |
| Keywords: | Acinetobacter
Bioemulsifier
Cleaning property
Emulsion
Human skin
Proteoglycan |
| Issue Date: | Jan-2010 |
| Publisher: | CSIR |
| Abstract: | Six Acinetobacter sp. isolated from healthy human skin were checked for the production of bioemulsifier. Optimization studies indicated that Luria Bertani broth pH 7 supplemented with calcium chloride (1%) was the optimum medium. Temperature at 37°C was optimum and inducer oils in the medium did not enhance bioemulsifier production. Partial purification of bioemulsifier and chemical analysis revealed that it is a proteoglycan with protein (53%), polysaccharide (43%) and lipid (2%). Maximum emulsification activity obtained was 400 EU/ml. Thin layer chromatography revealed the presence of mannose and rhamnose sugar and oleic and palmitic acids as parts of lipids. The yield obtained was 1.9 g / l. Reconstitution studies revealed that the protein and polysaccharide fractions together display 94.55% of emulsification activity. It was also noted that the bioemulsifier was stable for 72 hr at 37°C and displayed good cleaning property towards different oils. The partially purified bioemulsifier formed stable oil-in-water emulsions with plant oils. |
| Page(s): | 70-76 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(01) [January 2010]
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