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IJEB Vol.48(01) [January 2010] >


Title: Trans-differentiation of iris pigmented epithelial cells of Euphlyctis cyanophlyctis tadpoles into lens in vitro
Authors: Sharma, Manshi
Jangir, O P
Jhajharia, Sunita
Singh, Sumer
Singh, Vijay
Nagal, Amit
Keywords: Frog
Lens
Pigmented epithelial cells
Trans-differentiation
Issue Date: Jan-2010
Publisher: CSIR
Abstract: Meshed pigmented iris epithelium along with neural retina of tadpoles of the frog Euphlyctis cyanophlyctis were found to undergo dedifferentiation and subsequently transdifferentiate into lens in culture medium. During lag period, depigmentation (dedifferentiation) occurred in many cells. When culture became confluent 3-4 weeks after seeding tiny lens like structures differentiated from foci of cultured pigmented iris epithelium cells. The percentage of lens formation was higher in vitamin A treated cases. The culture system appears to be a suitable for investigating the changes occurred during trans-differentiation of pigmented epithelial cells into lens.
Page(s): 17-25
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.48(01) [January 2010]

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