Lipase-catalyzed regio- and stereoselective deacylation: Separation of anomers of peracylated <img src='/image/spc_char/alpha.gif' border=0>, <img src='/image/spc_char/beta.gif' border=0>-D-ribofuranosides

Abstract

Efficient regio- and stereoselective deacylation of acyloxy function involving C-5′ hydroxyl group of <img src='/image/spc_char/alpha.gif' border=0>-anomer over the other similar C-5′ acyl group of <img src='/image/spc_char/beta.gif' border=0>-anomer and acyl groups involving secondary hydroxyls in anomeric mixture of peracylates of D-ribose has been achieved during deacylation reaction mediated by Lipozyme^®<i> </i>TL IM (<i style="">Thermomyces lanuginosus</i> lipase immobilized on silica). This enzymatic methodology has been efficiently used for the separation of anomeric mixtures of peracylated <img src='/image/spc_char/alpha.gif' border=0>,<img src='/image/spc_char/beta.gif' border=0>-D-ribofuranosides.