Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/6728
Title: Antitrypanosomal effects of petroleum ether, chloroform and methanol extracts of <i style="">Artemisia maciverae </i>Linn.
Authors: Ene, A C
Atawodi, S E
Ameh, D A
Nnamani, C N
Apeh, Y E O
Keywords: Antitrypanosomal
<i style="">Artemisia maciverae</i>
Bioassay-guided assay
Issue Date: Dec-2009
Publisher: CSIR
Abstract: Petroleum ether, chloroform and methanol extracts of <i style="">A. maciverae</i> were studied <i style="">in vitro</i> and <i style="">in</i> <i style="">vivo</i> for activity against <i style="">Trypanosoma brucei brucei</i> in Swiss albino mice. Thereafter, the chloroform extract which showed the highest activity in both <i style="">in vitro</i> and <i style="">in vivo</i> assessments was subjected to bioassay-guided fractionation. The crude extracts and the fractions of the chloroform extract of <i style="">A. maciverae</i> were screened for phytochemicals and secondary metabolites. Combined fractions 54-57 of this extract showed the highest <i style="">in vitro</i> antitrypanosomal activity, and at 10 mg/kg body weight, this fraction cleared the parasitemia completely from <i style="">T. brucei brucei</i> infected Swiss albino mice after 7 days of treatment. There was no statistically significant difference in the level of parasitemia when the infected mice treated with this fraction was compared with the standard trypanocidal drug, diminal. The results of the phytochemical analysis showed that the crude extracts contained secondary metabolites like flavonoids, triterpenes, terpenoids, tannins, phlobatannins and alkaloids, while the active fraction contains only triterpenes and alkaloids. It can be inferred that fraction 54-57 contains the active component responsible for the high antitrypanosomal activity of the chloroform extract of <i style="">A. maciverae</i>.
Description: 981-986
URI: http://hdl.handle.net/123456789/6728
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.47(12) [December 2009]

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