NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(12) [December 2006] >


Title: Affinity purification and partial characterization of IgM-like immunoglobulins of African catfish, Clarias gariepinus (Burchell, 1822)
Authors: Rathore, Gaurav
Swaminathan, T Raja
Sood, Neeraj
Mishra, B N
Kapoor, D
Keywords: Chromatography
Clarias gariepinus
Fish
Immunoglobulin
Issue Date: Dec-2006
Publisher: CSIR
Abstract: IgM like macroglobulin from bovine serum albumin (BSA)-immunized African catfish C. gariepinus was purified by affinity chromatography and partially characterized. The molecular weight of this macroglobulin was 840 kDa, as estimated by gel filtration chromatography. Purified macroglobulin was analyzed using SDS-PAGE under reducing and non-reducing conditions. The molecular weight (MW) of heavy and light chain was 74.8 kDa and 27.2 kDa respectively, in presence of a reducing agent. In non-reducing SDS-PAGE, a single high MW band was observed representing tetrameric form.
Page(s): 1018-1021
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(12) [December 2006]

Files in This Item:

File Description SizeFormat
IJEB 44(12) 1018-1021.pdf126.47 kBAdobe PDFView/Open
 Current Page Visits: 947 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 588654 since 06-Feb-2009  Last updated on 18-Sep-2014Webmaster: nopr@niscair.res.in