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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(12) [December 2006] >
| Title: | Affinity purification and partial characterization of IgM-like immunoglobulins of African catfish, Clarias gariepinus (Burchell, 1822) |
| Authors: | Rathore, Gaurav
Swaminathan, T Raja
Sood, Neeraj
Mishra, B N
Kapoor, D |
| Keywords: | Chromatography
Clarias gariepinus
Fish
Immunoglobulin |
| Issue Date: | Dec-2006 |
| Publisher: | CSIR |
| Abstract: | IgM like macroglobulin from bovine serum
albumin (BSA)-immunized African catfish C.
gariepinus was purified by affinity chromatography and partially
characterized. The molecular weight of this macroglobulin was 840 kDa, as
estimated by gel filtration chromatography. Purified macroglobulin was analyzed
using SDS-PAGE under reducing and non-reducing conditions. The molecular weight
(MW) of heavy and light chain was 74.8 kDa and 27.2 kDa respectively, in
presence of a reducing agent. In non-reducing SDS-PAGE, a single high MW band
was observed representing tetrameric form. |
| Page(s): | 1018-1021 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(12) [December 2006]
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