Please use this identifier to cite or link to this item:
Title: Affinity purification and partial characterization of IgM-like immunoglobulins of African catfish, <i style="">Clarias gariepinus</i> (Burchell, 1822)<b style=""></b>
Authors: Rathore, Gaurav
Swaminathan, T Raja
Sood, Neeraj
Mishra, B N
Kapoor, D
Keywords: Chromatography
<i style="">Clarias gariepinus</i>
Issue Date: Dec-2006
Publisher: CSIR
Abstract: IgM like macroglobulin from bovine serum albumin (BSA)-immunized African catfish <i style="">C. gariepinus</i> was purified by affinity chromatography and partially characterized. The molecular weight of this macroglobulin was 840 kDa, as estimated by gel filtration chromatography. Purified macroglobulin was analyzed using SDS-PAGE under reducing and non-reducing conditions. The molecular weight (MW) of heavy and light chain was 74.8 kDa and 27.2 kDa respectively, in presence of a reducing agent. In non-reducing SDS-PAGE, a single high MW band was observed representing tetrameric form.
Description: 1018-1021
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(12) [December 2006]

Files in This Item:
File Description SizeFormat 
IJEB 44(12) 1018-1021.pdf126.47 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.