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Title: Effects of oxidizing and reducing agents on ovine pulmonary artery responses to nitric oxide donors, sodium nitroprusside and 3-morpholino-sydnonimine
Authors: Sardar, K K
Sarkar, S N
Bawankule, D U
Mishra, S K
Raviprakash, V
Keywords: Nitric oxide
Ovine pulmonary artery
Redox regulation
Issue Date: Dec-2006
Publisher: CSIR
Abstract: Nitrovasodilators-sodium nitroprusside (SNP; 10<sup>-9</sup>-10<sup>-4</sup> <i style="">M</i>) and 3-morpholino-sydnonimine (SIN-1; 10<sup>-9</sup>-10<sup>-4</sup> <i style="">M</i>) produced concentration-dependent relaxation of the fourth generation sheep pulmonary artery, preconstricted with 5-hydroxytryptamine (1 µ<i style="">M</i>). Oxidizing agents [oxidized glutathione (GSSG, 1 m<i style="">M</i>) and CuSO<sub>4</sub> (5 and 20 µ<i style="">M</i>)] and reducing agents [dithiothreitol (DTT, 0.1 m<i style="">M</i>), ascorbic acid (1 m<i style="">M</i>) and reduced glutathione (GSH, 1 m<i style="">M</i>)] caused opposite effects on nitric oxide (NO)-induced vasodilation in the artery. Ascorbic acid and GSH potentiated the NO responses, while GSSG and CuSO<sub>4</sub> inhibited relaxation caused by the nitrovasodilators. DTT, however, reduced the relaxant potency and efficacy of SNP and SIN-1. Pretreatment of the pulmonary artery strips with DTT (0.1 m<i style="">M</i>) inhibited SNP (10 µ<i style="">M</i>)-induced Na<sup>+-</sup>K<sup>+-</sup>ATPase activity, while ascorbic acid (1 m<i style="">M</i>) and GSH (1 m<i style="">M</i>) had no effect either on basal or SNP (10 µ<i style="">M</i>)-stimulated <sup>86</sup>Rb uptake, an index of Na<sup>+-</sup>K<sup>+-</sup>ATPase activity, in ovine pulmonary artery. The results suggest that reducing agents like ascorbic acid may have beneficial effect in improving the vascular function under oxidative stress.
Description: 964-970
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(12) [December 2006]

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