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Title: Effects of oxidizing and reducing agents on ovine pulmonary artery responses to nitric oxide donors, sodium nitroprusside and 3-morpholino-sydnonimine
Authors: Sardar, K K
Sarkar, S N
Bawankule, D U
Mishra, S K
Raviprakash, V
Keywords: Nitric oxide
Ovine pulmonary artery
Redox regulation
Issue Date: Dec-2006
Publisher: CSIR
Abstract: Nitrovasodilators-sodium nitroprusside (SNP; 10-9-10-4 M) and 3-morpholino-sydnonimine (SIN-1; 10-9-10-4 M) produced concentration-dependent relaxation of the fourth generation sheep pulmonary artery, preconstricted with 5-hydroxytryptamine (1 µM). Oxidizing agents [oxidized glutathione (GSSG, 1 mM) and CuSO4 (5 and 20 µM)] and reducing agents [dithiothreitol (DTT, 0.1 mM), ascorbic acid (1 mM) and reduced glutathione (GSH, 1 mM)] caused opposite effects on nitric oxide (NO)-induced vasodilation in the artery. Ascorbic acid and GSH potentiated the NO responses, while GSSG and CuSO4 inhibited relaxation caused by the nitrovasodilators. DTT, however, reduced the relaxant potency and efficacy of SNP and SIN-1. Pretreatment of the pulmonary artery strips with DTT (0.1 mM) inhibited SNP (10 µM)-induced Na+-K+-ATPase activity, while ascorbic acid (1 mM) and GSH (1 mM) had no effect either on basal or SNP (10 µM)-stimulated 86Rb uptake, an index of Na+-K+-ATPase activity, in ovine pulmonary artery. The results suggest that reducing agents like ascorbic acid may have beneficial effect in improving the vascular function under oxidative stress.
Page(s): 964-970
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(12) [December 2006]

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