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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(12) [December 2006] >
| Title: | Effects of oxidizing and reducing agents on ovine pulmonary artery responses to nitric oxide donors, sodium nitroprusside and 3-morpholino-sydnonimine |
| Authors: | Sardar, K K
Sarkar, S N
Bawankule, D U
Mishra, S K
Raviprakash, V |
| Keywords: | Nitric oxide
Ovine pulmonary artery
Redox regulation |
| Issue Date: | Dec-2006 |
| Publisher: | CSIR |
| Abstract: | Nitrovasodilators-sodium nitroprusside (SNP;
10-9-10-4 M)
and 3-morpholino-sydnonimine (SIN-1; 10-9-10-4 M)
produced concentration-dependent relaxation of the fourth generation sheep
pulmonary artery, preconstricted with 5-hydroxytryptamine (1 µM). Oxidizing agents [oxidized glutathione (GSSG, 1 mM) and CuSO4 (5 and 20 µM)] and reducing agents [dithiothreitol (DTT, 0.1 mM), ascorbic acid (1 mM) and reduced glutathione (GSH, 1 mM)] caused opposite effects on nitric
oxide (NO)-induced vasodilation in the artery. Ascorbic acid and GSH
potentiated the NO responses, while GSSG and CuSO4 inhibited
relaxation caused by the nitrovasodilators. DTT, however, reduced the relaxant
potency and efficacy of SNP and SIN-1. Pretreatment of the pulmonary artery
strips with DTT (0.1 mM) inhibited
SNP (10 µM)-induced Na+-K+-ATPase
activity, while ascorbic acid (1 mM)
and GSH (1 mM) had no effect either
on basal or SNP (10 µM)-stimulated 86Rb
uptake, an index of Na+-K+-ATPase activity, in ovine
pulmonary artery. The results suggest that reducing agents like ascorbic acid
may have beneficial effect in improving the vascular function under oxidative
stress. |
| Page(s): | 964-970 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(12) [December 2006]
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