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Title: Isoflavonoids production in callus culture of Pueraria tuberosa, the Indian kudzu
Authors: Vaishnav, Kamlesh
Goyal, Shaily
Ramawat, K G
Keywords: Callus culture
Isoflavonoids production
Pueraria tuberosa
Issue Date: Dec-2006
Publisher: CSIR
Abstract: Isoflavonoid contents of different plant parts and callus tissues of the Indian Kudzu, Pueraria tuberosa (Roxb.ex.Willd.) DC are presented. The initial cultures were slow growing, associated with browning of the tissues. The production of four isoflavonoids (puerarin, genistin, genistein and daidzein) in the callus cultures of P. tuberosa was studied by manipulating the plant growth regulators and sucrose concentration in the medium. Organogenesis was not recorded in callus on any of these treatments. Tuber and stem accumulated puerarin, a glycoside of daidzein, at high amounts, 0.65% and 0.054% respectively. However, the daidzein content of the callus tissues grown on Murashige and Skoog medium containing BA (20.9 ยตM) and sucrose (60 gl-1) was significantly higher (0.056%) than in vivo plant material (0.02%) and other comparable culture systems like Genista and Pueraria lobata.
Page(s): 1012-1017
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(12) [December 2006]

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