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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(12) [December 2006] >
| Title: | Isoflavonoids production in callus culture of Pueraria tuberosa, the Indian kudzu |
| Authors: | Vaishnav, Kamlesh
Goyal, Shaily
Ramawat, K G |
| Keywords: | Callus culture
Isoflavonoids production
Pueraria tuberosa |
| Issue Date: | Dec-2006 |
| Publisher: | CSIR |
| Abstract: | Isoflavonoid contents of different plant
parts and callus tissues of the Indian Kudzu, Pueraria tuberosa (Roxb.ex.Willd.) DC are presented. The initial cultures
were slow growing, associated with browning of the tissues. The production of
four isoflavonoids (puerarin, genistin, genistein and daidzein) in the callus
cultures of P. tuberosa was studied
by manipulating the plant growth regulators and sucrose concentration in the
medium. Organogenesis was not recorded in callus on any of these treatments.
Tuber and stem accumulated puerarin, a glycoside of daidzein, at high amounts,
0.65% and 0.054% respectively. However, the daidzein content of the callus tissues
grown on Murashige and Skoog medium containing BA (20.9 ยตM) and sucrose (60 gl-1) was significantly higher
(0.056%) than in vivo plant material
(0.02%) and other comparable culture systems like Genista and Pueraria lobata. |
| Page(s): | 1012-1017 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(12) [December 2006]
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