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Title: Isoflavonoids production in callus culture of <i style="">Pueraria tuberosa,</i> the Indian kudzu
Authors: Vaishnav, Kamlesh
Goyal, Shaily
Ramawat, K G
Keywords: Callus culture
Isoflavonoids production
<i style="">Pueraria tuberosa</i>
Issue Date: Dec-2006
Publisher: CSIR
Abstract: Isoflavonoid contents of different plant parts and callus tissues of the Indian Kudzu, <i style="">Pueraria tuberosa</i> (Roxb.ex.Willd.) DC are presented. The initial cultures were slow growing, associated with browning of the tissues. The production of four isoflavonoids (puerarin, genistin, genistein and daidzein) in the callus cultures of <i style="">P. tuberosa</i> was studied by manipulating the plant growth regulators and sucrose concentration in the medium. Organogenesis was not recorded in callus on any of these treatments. Tuber and stem accumulated puerarin, a glycoside of daidzein, at high amounts, 0.65% and 0.054% respectively. However, the daidzein content of the callus tissues grown on Murashige and Skoog medium containing BA (20.9 µ<i style="">M</i>) and sucrose (60 gl<sup>-1</sup>) was significantly higher (0.056%) than <i style="">in vivo</i> plant material (0.02%) and other comparable culture systems like <i style="">Genista </i>and<i style=""> Pueraria lobata</i>.
Description: 1012-1017
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(12) [December 2006]

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