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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(12) [December 2006] >
| Title: | Cytogenetic effects of a mixture of selected metals following subchronic exposure through drinking water in male rats |
| Authors: | Jadhav, S H
Sarkar, S N
Tripathi, H C |
| Keywords: | Genotoxicity
Groundwater contaminants
Metal mixture
Oxidative stress
Rat |
| Issue Date: | Dec-2006 |
| Publisher: | CSIR |
| Abstract: | The current study examines the genotoxic
effects of subchronic exposure via drinking water to a mixture of eight metals
(arsenic, cadmium, lead, mercury, chromium, nickel, manganese and iron) found
as contaminants of water sources in different parts of India and its possible
association with oxidative stress. Male rats were exposed to the mixture at 0,
1, 10 and 100 times the mode concentration of each metal daily for 90 days.
Another dose group at concentration equivalent to maximum permissible limit
(MPL) for each metal and a reference group given ip cyclophosphamide were incorporated.
The mixture at 100×level significantly increased
chromosomal aberrations and micronuclei induction (2.4 folds) in bone marrow
cells and reduced the ratio of polychromatic to normochromatic erythrocytes by
25%. The mixture significantly increased sister chromatid exchange in bone marrow
(1.67 and 2.3
folds) and spleen (1.57 and 1.98 folds) cells with both 10× and 100× doses. Cyclophosphamide was more potent than the mixture in causing
cytogenetic damage in these parameters. In rat spleen, the mixture at 10× and 100× doses
caused dose-dependent increase in lipid peroxidation (25.95 and 52.71%) and
decrease in the activities of superoxide dismutase (20.36 and 40.62%), catalase
(18.24 and 35.50%), glutathione peroxidase (22.33 and 36.12%) and glutathione
reductase (19.22 and 31.35%) and in the level of GSH (19.76 and 35.15%). The
results suggest that the mixture induced genotoxicity in rat bone marrow and
spleen cells at concentrations relatively higher than that found in groundwater
sources and the genotoxic effect could relate to induction of oxidative stress.
However, observations with lower doses indicate that additive or synergistic
interactions following exposure to metal components at MPL levels or at mode concentrations
of contemporary groundwater levels in India may not result in clastogenicity in
male rats. |
| Page(s): | 997-1005 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(12) [December 2006]
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