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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(12) [December 2006] >
| Title: | Phage typing of indigenous soybean-rhizobia and relationship of a phage group strains for their asymbiotic and symbiotic nitrogen fixation |
| Authors: | Appunu, C
Dhar, B |
| Keywords: | Bradyrhizobia
Ex planta nitrogenase activity
Phage typing
Soybean
Symbiotic effectiveness |
| Issue Date: | Dec-2006 |
| Publisher: | CSIR |
| Abstract: | A total of 354 indigenous bradyrhizobia were
isolated from soybean nodules collected from five major crop grown regions.
Host-specific 12 phages, each active on particular strains were selected.
Factors, which influence the interaction between the host and phage, were
examined. Four different types of plaques were detected. Nearly 17% of isolates
were found resistant to all phages. Phage sensitivity patterns revealed a total
of 32 distinct phage genotype groups. Different
set of phage combinations expressed variation in specificity for parasitizing
against particular group of rhizobia. Distributions of isolates in each phage
types differed markedly between regions. Interestingly, nine strains belonging
to phage group 16 exhibited high ex
planta nitrogenase activity in culture. However, no correlation could be
established between high ex planta
nitrogenase activity and their symbiotic effectiveness with soybean cultivars.
Soybean cv. JS335 showed relatively superior performance than Bragg and Lee
with indigenous bradyrhizobial strains. Phage typing revealed the existence of
large genetic diversity among native rhizobia and selection of the superior
bradyrhizobial strains can also be possible for a given soil-climate-cultivar
complex. |
| Page(s): | 1006-1011 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(12) [December 2006]
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