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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(12) [December 2006] >
| Title: | PEG-induced fusion of phosphatidylcholine-liposomes with protoplasts and post-fusion evaluation of plating efficiency and enrichment in plasmamembrane phosphatidylcholine of protoplasts in Datura innoxia Mill |
| Authors: | Manoharan, Kumariah
Prasad, Rajendra
Guha-Mukherjee, Sipra |
| Keywords: | Datura innoxia Mill
Liposomes
PEG
Phosphatidylcholine
Plasmamembrane phospholipids
Plating efficiency
Protoplasts |
| Issue Date: | Dec-2006 |
| Publisher: | CSIR |
| Abstract: | Liposomes entrapping fluorescein diacetate
were fused with protoplasts of Datura
innoxia Mill by employing polyethylene glycol (PEG) as the fusogen. Factors
that influence liposome-protoplast fusion were optimized as a function of
PEG-concentration and incubation duration, liposome composition and surface
charge and liposome:protoplast ratio. Phosphatidylcholine-liposomes were found
ideal for the objectives of the study. Fusion index based on per cent
fluorescing protoplasts varied among the protoplast types. PEG-incubation
duration in the fusion assay and growth ability of protoplasts to form
microcalli subsequent to liposome-protoplast fusion was determined based on
protoplast plating-efficiency. Plating efficiency of post-fusion protoplasts
increased due to incorporation of liposome-phosphatidylcholine in the
plasmamembrane of protoplasts. Results are discussed in relation to the
application of liposome-protoplast fusion system in selective modification of
plasmamembrane phospholipids of protoplasts. |
| Page(s): | 955-963 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(12) [December 2006]
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