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IJEB Vol.44(12) [December 2006] >


Title: PEG-induced fusion of phosphatidylcholine-liposomes with protoplasts and post-fusion evaluation of plating efficiency and enrichment in plasmamembrane phosphatidylcholine of protoplasts in Datura innoxia Mill
Authors: Manoharan, Kumariah
Prasad, Rajendra
Guha-Mukherjee, Sipra
Keywords: Datura innoxia Mill
Liposomes
PEG
Phosphatidylcholine
Plasmamembrane phospholipids
Plating efficiency
Protoplasts
Issue Date: Dec-2006
Publisher: CSIR
Abstract: Liposomes entrapping fluorescein diacetate were fused with protoplasts of Datura innoxia Mill by employing polyethylene glycol (PEG) as the fusogen. Factors that influence liposome-protoplast fusion were optimized as a function of PEG-concentration and incubation duration, liposome composition and surface charge and liposome:protoplast ratio. Phosphatidylcholine-liposomes were found ideal for the objectives of the study. Fusion index based on per cent fluorescing protoplasts varied among the protoplast types. PEG-incubation duration in the fusion assay and growth ability of protoplasts to form microcalli subsequent to liposome-protoplast fusion was determined based on protoplast plating-efficiency. Plating efficiency of post-fusion protoplasts increased due to incorporation of liposome-phosphatidylcholine in the plasmamembrane of protoplasts. Results are discussed in relation to the application of liposome-protoplast fusion system in selective modification of plasmamembrane phospholipids of protoplasts.
Page(s): 955-963
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(12) [December 2006]

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