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Title: Mutation in <i style="">mexR</i>-gene leading to drug resistance in corneal keratitis in human
Authors: G, Suman
M, Khan
K, Sabitha
Jamil, Kaiser
Keywords: Antibiotics
Efflux pumps
<i style="">Pseudomonas aeruginosa</i>
Issue Date: Nov-2006
Publisher: CSIR
Abstract: The present study deals with the genetic polymorphism of the <i style="">mexR</i> gene which is involved in the resistance to drugs like ciprofloxacin.&nbsp; Mutations in <i style="">mexR</i> result in increased resistance to multiple antibiotics due to overexpression of this efflux system. The MexR product contains 147 amino acids with a molecular mass of 16,964 Da. We detected 28 point mutations in 14 samples from corneal scraping infected with <i style="">Pseudomonas aeruginosa,</i> which<i style=""> </i>were screened for ciprofloxacin resistance. Twenty four were silent mutations and four missense mutations. Mapping these mutations was done by using in silico methods on the protein 3D- structure obtained from PDB database, localized at 3 specific sites. Single amino acid changes (mutations) may influence MexR stability or its ability to dimerise, and thus result in the conformation changes at the DNA-binding domain, of the structure. Hence it is concluded that the mutations in the DNA binding domain of <i style="">mexR</i> gene could be one of the factors contributing to the possible drug resistance in these patients.
Description: 929-936
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(11) [November 2006]

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