Please use this identifier to cite or link to this item:
|Title:||Mutation in <i style="">mexR</i>-gene leading to drug resistance in corneal keratitis in human|
<i style="">Pseudomonas aeruginosa</i>
|Abstract:||The present study deals with the genetic polymorphism of the <i style="">mexR</i> gene which is involved in the resistance to drugs like ciprofloxacin. Mutations in <i style="">mexR</i> result in increased resistance to multiple antibiotics due to overexpression of this efflux system. The MexR product contains 147 amino acids with a molecular mass of 16,964 Da. We detected 28 point mutations in 14 samples from corneal scraping infected with <i style="">Pseudomonas aeruginosa,</i> which<i style=""> </i>were screened for ciprofloxacin resistance. Twenty four were silent mutations and four missense mutations. Mapping these mutations was done by using in silico methods on the protein 3D- structure obtained from PDB database, localized at 3 specific sites. Single amino acid changes (mutations) may influence MexR stability or its ability to dimerise, and thus result in the conformation changes at the DNA-binding domain, of the structure. Hence it is concluded that the mutations in the DNA binding domain of <i style="">mexR</i> gene could be one of the factors contributing to the possible drug resistance in these patients.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.44(11) [November 2006]|
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.