|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(11) [November 2006] >
| Title: | Mutation in mexR-gene leading to drug resistance in corneal keratitis in human |
| Authors: | G, Suman
M, Khan
K, Sabitha
Jamil, Kaiser |
| Keywords: | Antibiotics
Efflux pumps
Mutations
Pseudomonas aeruginosa |
| Issue Date: | Nov-2006 |
| Publisher: | CSIR |
| Abstract: | The present study deals with the
genetic polymorphism of the mexR gene
which is involved in the resistance to drugs like ciprofloxacin. Mutations in mexR result in increased resistance to multiple antibiotics due to
overexpression of this efflux system. The MexR product contains 147 amino acids
with a molecular mass of 16,964 Da. We detected 28 point mutations in 14
samples from corneal scraping infected with Pseudomonas
aeruginosa, which were screened
for ciprofloxacin resistance. Twenty four were silent mutations and four
missense mutations. Mapping these mutations was done by using in silico methods
on the protein 3D- structure obtained from PDB database, localized at 3
specific sites. Single amino acid changes (mutations) may influence MexR
stability or its ability to dimerise, and thus result in the conformation
changes at the DNA-binding domain, of the structure. Hence it is concluded that
the mutations in the DNA binding domain of mexR
gene could be one of the factors contributing to the possible drug resistance
in these patients. |
| Page(s): | 929-936 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(11) [November 2006]
|
|
