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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(10) [October 2006] >
| Title: | Protective effect of ethanolic and water extracts of sea buckthorn (Hippophae rhamnoides L.) against the toxic effects of mustard gas |
| Authors: | Vijayaraghavan, R
Gautam, Anshoo
Kumar, Om
Pant, S C
Sharma, Manoj
Singh, Seema
Kumar, H T Satish
Singh, Anand Kumar
Nivsarkar, Manisha
Kaushik, M P
Sawhney, R C
Chaurasia, O P
Prasad, G B K S |
| Keywords: | Glutathione
Hippophae rhamnoides
Malondialdehyde
Oxidative stress
Sulphur mustard |
| Issue Date: | Oct-2006 |
| Publisher: | CSIR |
| Abstract: | Ethanolic extract of H. rhamnoides L. leaf (HL-EOH), water and ethanolic extract of H. rhamnoides fruit (HF-W and HF-EOH),
and H. rhamnoides flavone from fruit
(HR-flavone) were evaluated against percutaneously administered sulphur mustard
(SM), a chemical warfare agent. The animals administered with SM (9.7, 19.3 and
38.7 mg/kg) died at various days depending upon the dose and there was a
significant reduction in the body weight. The H. rhamnoides extracts (1g/kg; 3 doses; po) significantly protected
the lethality, with a protective index of 2.4, 1.7, 1.7 and 2.2 for HL-EOH,
HF-W, HF-EOH and HR-flavone respectively. Reduced glutathione (GSH) and
oxidized glutalthione (GSSG) levels were reduced, and malondialdehyde (MDA) was
elevated after percutaneous administration of SM. Oral administration of HL-EOH
and HR-flavone significantly protected the body weight loss. Recovery in the
levels of GSH, GSSG and MDA were also observed following oral administration of
HL-EOH and HR-flavone. All the extracts were non-toxic and the LD50
was more than 5 g/kg. The present study shows that percutaneous administration
of SM induces oxidative stress and ethanolic extract of leaf of H. rhamnoides and H. rhamnoides flavone from fruit can significantly protect it. |
| Page(s): | 821-831 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(10) [October 2006]
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