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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(10) [October 2006] >
| Title: | DNA isolation from goat blood using different brands of household detergents and its downstream application |
| Authors: | Kumar, O Suneel
Sharma, M K
Singh, Dheer |
| Keywords: | Aromatase
Detergent
DNA isolation
PCR |
| Issue Date: | Oct-2006 |
| Publisher: | CSIR |
| Abstract: | Rapid isolation of DNA from goat blood using
different brands of detergents available in Indian market, is reported. The
integrity and efficiency of these DNA preparations were compared with genomic DNA
isolated by a standard kit (Flexi gene DNA kit), using amplification of exon 2
of CYP19 (aromatase) gene. The similar and significant amplification of this
gene was obtained using genomic DNA isolated by kit and various detergents.
However, among the detergents used, the Rin and Ezee were found to be the best
to get DNA of high purity comparable to that obtained by kit. |
| Page(s): | 852-854 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(10) [October 2006]
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