NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(10) [October 2006] >


Title: DNA isolation from goat blood using different brands of household detergents and its downstream application
Authors: Kumar, O Suneel
Sharma, M K
Singh, Dheer
Keywords: Aromatase
Detergent
DNA isolation
PCR
Issue Date: Oct-2006
Publisher: CSIR
Abstract: Rapid isolation of DNA from goat blood using different brands of detergents available in Indian market, is reported. The integrity and efficiency of these DNA preparations were compared with genomic DNA isolated by a standard kit (Flexi gene DNA kit), using amplification of exon 2 of CYP19 (aromatase) gene. The similar and significant amplification of this gene was obtained using genomic DNA isolated by kit and various detergents. However, among the detergents used, the Rin and Ezee were found to be the best to get DNA of high purity comparable to that obtained by kit.
Page(s): 852-854
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(10) [October 2006]

Files in This Item:

File Description SizeFormat
IJEB 44(10) 852-854.pdf293.59 kBAdobe PDFView/Open
 Current Page Visits: 545 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 602124 since 06-Feb-2009  Last updated on 17-Oct-2014Webmaster: nopr@niscair.res.in