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Title: DNA isolation from goat blood using different brands of household detergents and its downstream application
Authors: Kumar, O Suneel
Sharma, M K
Singh, Dheer
Keywords: Aromatase;Detergent;DNA isolation;PCR
Issue Date: Oct-2006
Publisher: CSIR
Abstract: Rapid isolation of DNA from goat blood using different brands of detergents available in Indian market, is reported. The integrity and efficiency of these DNA preparations were compared with genomic DNA isolated by a standard kit (Flexi gene DNA kit), using amplification of exon 2 of CYP19 (aromatase) gene. The similar and significant amplification of this gene was obtained using genomic DNA isolated by kit and various detergents. However, among the detergents used, the Rin and Ezee were found to be the best to get DNA of high purity comparable to that obtained by kit.
Page(s): 852-854
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(10) [October 2006]

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