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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(10) [October 2006] >
| Title: | Optimization of nutritional requirements for gentamicin production by Micromonospora echinospora |
| Authors: | M, Himabindu
Jetty, Annapurna |
| Keywords: | Gentamicin production
Micromonospora echinospora
Nutritional requirement |
| Issue Date: | Oct-2006 |
| Publisher: | CSIR |
| Abstract: | Effect of various fermentation media, carbon
sources, nitrogen sources, phosphate concentration and culture requirements
includes inoculum levels and age were determined on gentamicin production and
biomass dry weight production for Micromonospora
echinospora, a gentamicin producing strain. Of the substrates tested,
starch as a sole carbon source promoted maximal gentamicin production, while
maltose promoted maximal growth. Yeast extract as a sole nitrogen source
promoted maximal growth, while soyabean meal for gentamicin production.
Increasing phosphate concentration enhanced gentamicin production and observed
optimum production at 1.2g/l (6% v/v) of phosphate having 72 h old inoculum in
the medium. Highest gentamicin production was obtained after cultivation with
shaking for 120 h in a medium containing starch 0.75%(w/v), soyabean meal 0.5%,
K2HPO4 0.12%, CaCO3 0.4%, FeSO4 0.003%
and CoCl2 0.0001%. The gentamicin production was 1.2-fold in this
medium as compared to basal medium. |
| Page(s): | 842-848 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(10) [October 2006]
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