Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/6617
Title: Optimization of nutritional requirements for gentamicin production by Micromonospora echinospora
Authors: M, Himabindu
Jetty, Annapurna
Keywords: Gentamicin production
Micromonospora echinospora
Nutritional requirement
Issue Date: Oct-2006
Publisher: CSIR
Abstract: Effect of various fermentation media, carbon sources, nitrogen sources, phosphate concentration and culture requirements includes inoculum levels and age were determined on gentamicin production and biomass dry weight production for Micromonospora echinospora, a gentamicin producing strain. Of the substrates tested, starch as a sole carbon source promoted maximal gentamicin production, while maltose promoted maximal growth. Yeast extract as a sole nitrogen source promoted maximal growth, while soyabean meal for gentamicin production. Increasing phosphate concentration enhanced gentamicin production and observed optimum production at 1.2g/l (6% v/v) of phosphate having 72 h old inoculum in the medium. Highest gentamicin production was obtained after cultivation with shaking for 120 h in a medium containing starch 0.75%(w/v), soyabean meal 0.5%, K2HPO4 0.12%, CaCO3 0.4%, FeSO4 0.003% and CoCl2 0.0001%. The gentamicin production was 1.2-fold in this medium as compared to basal medium.
Description: 842-848
URI: http://hdl.handle.net/123456789/6617
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(10) [October 2006]

Files in This Item:
File Description SizeFormat 
IJEB 44(10) 842-848.pdf132.1 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.