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Title: Optimization of nutritional requirements for gentamicin production by <i style="">Micromonospora echinospora</i>
Authors: M, Himabindu
Jetty, Annapurna
Keywords: Gentamicin production
<i style="">Micromonospora echinospora</i>
Nutritional requirement
Issue Date: Oct-2006
Publisher: CSIR
Abstract: Effect of various fermentation media, carbon sources, nitrogen sources, phosphate concentration and culture requirements includes inoculum levels and age were determined on gentamicin production and biomass dry weight production for <i style="">Micromonospora echinospora</i>, a gentamicin producing strain. Of the substrates tested, starch as a sole carbon source promoted maximal gentamicin production, while maltose promoted maximal growth. Yeast extract as a sole nitrogen source promoted maximal growth, while soyabean meal for gentamicin production. Increasing phosphate concentration enhanced gentamicin production and observed optimum production at 1.2g/l (6% v/v) of phosphate having 72 h old inoculum in the medium. Highest gentamicin production was obtained after cultivation with shaking for 120 h in a medium containing starch 0.75%(w/v), soyabean meal 0.5%, K<sub>2</sub>HPO<sub>4 </sub>0.12%, CaCO<sub>3 </sub>0.4%, FeSO<sub>4 </sub>0.003% and CoCl<sub>2</sub> 0.0001%. The gentamicin production was 1.2-fold in this medium as compared to basal medium.
Description: 842-848
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(10) [October 2006]

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