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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(10) [October 2006] >
| Title: | Heat stress induced apoptosis in BC-8 cells derived from AK-5 tumor involves downregulation of Bcl-2 and generation of reactive oxygen species |
| Authors: | Khar, Ashok
Kumari, A Leela
Pardhasaradhi, B V V
Varalakshmi, Ch
Rangaraj, Nandini |
| Keywords: | AK-5 tumor
Apoptosis
Bcl-2
Heat shock
ROS |
| Issue Date: | Oct-2006 |
| Publisher: | CSIR |
| Abstract: |
BC-8, a rat histiocytoma undergoes apoptosis after
heat shock, which is due to lack of an effective heat shock response. Heat
shock induced generation of free radicals, which in turn are involved in the
induction of apoptotic death in BC-8 cells. Treatment of BC-8 cells with
N-acetylcysteine partially inhibited the heat induced apoptosis. Introduction
of Bcl-2 gene in these cells did not protect them from apoptotic death, whereas
transfection with hsp-70 gene did render these cells resistant to heat induced
apoptosis transiently. Heat shock also downregulated the expression of Bcl-2
and p53 in these cells. These observations suggested that the heat shock induced
apoptosis was mediated through reactive oxygen species and controlled upstream
of Bcl-2 check point.
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| Page(s): | 802-808 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(10) [October 2006]
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