Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/6600
Title: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"> Efficient protocols for <i style="">in vitro</i> regeneration of <i style="">Pennisetum glaucum </i>(L) Br. </smarttagtype></smarttagtype>
Authors: Arockiasamy, S
Rani, S Sahaya
Ignacimuthu, S
Melchias, G
Keywords: Bajra
Leaf base
Mature embryo
Pearl millet
Poaceae
Regeneration
Issue Date: Sep-2006
Publisher: CSIR
Abstract: A system was developed for <i>in vitro</i> regeneration of <i style="">Pennisetum glaucum</i> through organogenesis and somatic embryogenesis. Mature embryo and leaf base explants of <i style="">Pennisetum glaucum </i>(L) Br. cv HH B60 (Poaceae) were cultured on Murashige and Skoog agar medium supplemented with 11.3 µ<i>M</i> of 2,4-D for callus induction. Embryogenic calli were induced within eight weeks. Percentage of callus induction and somatic embryogenesis was significantly higher in mature embryo than leaf base explants. Maximum shoot regeneration was obtained via organogenesis on MS medium supplemented with 4.43 µ<i>M </i>of BAP and 4.64 µ<i>M</i> of kinetin from the calli of both the explants. The frequency of plant regeneration through somatic embryogenesis was comparatively lower than organogenesis. Regeneration frequency was higher in mature embryo explants than leaf base explants. The shoots regenerated via organogenesis were elongated and rooted efficiently on MS medium supplemented with IBA (0.49 µ<i>M</i>). The rooted plantlets were hardened and transferred to soil.
Description: 757-761
URI: http://hdl.handle.net/123456789/6600
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(09) [September 2006]

Files in This Item:
File Description SizeFormat 
IJEB 44(9) 757-761.pdf129.03 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.