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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(09) [September 2006] >
| Title: | Efficient protocols for in vitro regeneration of Pennisetum glaucum (L) Br. |
| Authors: | Arockiasamy, S
Rani, S Sahaya
Ignacimuthu, S
Melchias, G |
| Keywords: | Bajra
Leaf base
Mature embryo
Pearl millet
Poaceae
Regeneration |
| Issue Date: | Sep-2006 |
| Publisher: | CSIR |
| Abstract: | A system was developed for in vitro regeneration of Pennisetum glaucum through organogenesis
and somatic embryogenesis. Mature embryo and leaf base explants of Pennisetum glaucum (L) Br. cv HH B60 (Poaceae) were
cultured on Murashige and Skoog agar medium supplemented with 11.3 µM of
2,4-D for callus induction. Embryogenic calli were induced within eight weeks.
Percentage of callus induction and somatic embryogenesis was significantly
higher in mature embryo than leaf base explants. Maximum shoot regeneration was
obtained via organogenesis on MS medium supplemented with 4.43 µM of BAP
and 4.64 µM of kinetin from the calli of both the explants. The
frequency of plant regeneration through somatic embryogenesis was comparatively
lower than organogenesis. Regeneration frequency was higher in mature embryo
explants than leaf base explants. The shoots regenerated via organogenesis were
elongated and rooted efficiently on MS medium supplemented with IBA (0.49 µM).
The rooted plantlets were hardened and transferred to soil. |
| Page(s): | 757-761 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(09) [September 2006]
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