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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(09) [September 2006] >
| Title: | Prepatent detection of Fasciola gigantica infection in bovine calves using metacercarial antigen |
| Authors: | Velusamy, R
Singh, B P
Ghosh, S
Chandra, D
Raina, O K
Gupta, S C
Jayraw |
| Keywords: | Bovine calves
ELISA
Fasciola gigantica
Metacercarial antigen |
| Issue Date: | Sep-2006 |
| Publisher: | CSIR |
| Abstract: | Metacercarial antigen of Fasciola gigantica was evaluated for
early immunodiagnosis of experimental bovine fasciolosis using ELISA and Western
blot. In ELISA, the experimental F.
gigantica infection was detected as early as 2 weeks post-infection (WPI).
The gradual increasing trend of antibody level was observed from 2 to 7 WPI,
followed by a plateau, which was maintained up to 14 WPI. In Western blot, sera
from experimentally infected calves recognized one distinct polypeptide of 21
kDa in fractionated metacercarial antigen as early as 10 th day post
infection. From 2 WPI, more polypeptide bands were reacting. Recognition of
these protein bands persisted till the end of the experiment (14 WPI). Cattle
sera collected from the field showed 34.5% seroprevalence of fasciolosis by
ELISA using MAg. Comparative immunoblot studies of metacercarial antigen with
anti-Gigantocotyle explanatum and anti-Paramphistomum
epiclitum sera revealed that 21 and 25 kDa polypeptides of metacercarial
antigen did not cross-react with any of these sera and appear to be unique to
F. gigantica and having the desirable
qualities of early and specific immunodiagnosis. |
| Page(s): | 749-753 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(09) [September 2006]
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