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Title: Differential role of MAP kinase isoforms in malachite green transformed Syrian hamster embryo fibroblasts in culture
Authors: Bose, Bipasha
Gour, Rekha R
Motiwale, Leena
Rao, K V K
Keywords: Embryo cell
Increased S-phase
Malachite green
Malignant transformation
MAP kinases
Syrian hamster embryo cells
Issue Date: Sep-2006
Publisher: CSIR
Abstract: Malachite green (MG) induces DNA damage and malignant transformation of Syrian hamster embryo (SHE) cells in primary culture. In the present study, we have studied the role of all the three isoforms of mitogen activated protein (MAP) kinases i.e. ERK (extracellular regulated kinase), JNK (JUN- N- terminal kinase) and p38 kinase during transformation of SHE cells by MG. The results showed that transformed cells were associated with a decreased expression of phosphoactive ERK and JNK and increased expression of p38 kinase as evident from the Western blot, immunofluorescence and flow cytometry studies. Also, a persistent nuclear localization of p38 kinase was observed in the transformed cells. The present study indicated that p38 kinase was present at higher levels and seemed to be associated with transformation, which suggested that inhibitors of p38 kinase could serve in general as potential agents for selective cancer therapy.
Page(s): 693-698
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(09) [September 2006]

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