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IJEB Vol.44(09) [September 2006] >


Title: Catalytic and regulatory properties of sulphur metabolizing enzymes in cyanobacterium Synechococcus elongatus PCC 7942
Authors: Jain, Anuradha
Verma, Deepti
Bagchi, Divya
Keywords: Catalytic property
Cyanobacterium
Regulatory property
Sulphur metabolizing enzyme
Synechococcus elongatus
Issue Date: Sep-2006
Publisher: CSIR
Abstract: Synechococcus elongatus PCC 7942 was able to grow with several S sources. The sulphur metabolizing enzymes viz. ATP sulphurylase, cysteine synthase, thiosulphate reductase and L- and D-cysteine desulphydrases were regulated by sulphur sources, particularly by sulphur amino acids and organic sulphate esters. Sulphur starvation reduced ATP sulphurylase and cysteine synthase whereas reduced glutathione appreciated Cys degradation activity. With partially purified enzymes apparent Km values for sulphate, ATP, D- and L-Cys, thiosulphate, sulphide and O-acetyl serine were in a range of 12-50 ยตM. p-Nitrophenyl sulphate inhibited ATP sulphurylase competitively. Met was a feedback inhibitor of several key enzymes.
Page(s): 767-772
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(09) [September 2006]

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