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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(09) [September 2006] >
| Title: | Catalytic and regulatory properties of sulphur metabolizing enzymes in cyanobacterium Synechococcus elongatus PCC 7942 |
| Authors: | Jain, Anuradha
Verma, Deepti
Bagchi, Divya |
| Keywords: | Catalytic property
Cyanobacterium
Regulatory property
Sulphur metabolizing enzyme
Synechococcus elongatus |
| Issue Date: | Sep-2006 |
| Publisher: | CSIR |
| Abstract: | Synechococcus
elongatus PCC 7942 was able to grow with several S
sources. The sulphur metabolizing enzymes viz.
ATP sulphurylase, cysteine synthase, thiosulphate reductase and L- and D-cysteine desulphydrases were regulated by
sulphur sources, particularly by sulphur amino acids and organic sulphate
esters. Sulphur starvation reduced ATP sulphurylase and cysteine synthase whereas
reduced glutathione appreciated Cys degradation activity. With partially
purified enzymes apparent Km values
for sulphate, ATP, D- and L-Cys,
thiosulphate, sulphide and O-acetyl
serine were in a range of 12-50 ยตM. p-Nitrophenyl sulphate inhibited ATP sulphurylase
competitively. Met was a feedback inhibitor of several key enzymes. |
| Page(s): | 767-772 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(09) [September 2006]
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