NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Chemistry -Section B (IJC-B) >
IJC-B Vol.46B [2007] >
IJC-B Vol.46B(07) [July 2007] >


Title: Simple, economical and flexible apparatus for solid phase peptide synthesis
Authors: Satyanarayana, Kota
Kumar, K V R C Rajesh
Venkanna, Ch
Keywords: Peptide synthesis
Solid phase
Manual solid phase synthesis
Two-vessel solid phase peptide synthesis
Enkephalins
Issue Date: Jul-2007
Publisher: CSIR
Abstract: Solid phase peptide synthesis basically involves repetitive deblocking and coupling reactions. In order to make these repetitive operations fast and convenient, the deblocking and coupling reactions are carried out in specially designed apparatus, which are called solid phase peptide synthesis apparatus. These apparatus are expensive, hence unaffordable for many laboratories. In addition, these apparatus lack the operational flexibility for varying reaction parameters such as reaction time, temperature, pressure, sonication, microwave irradiation, etc. In order to make peptide synthesis convenient and flexible with respect to reaction conditions and affordable for many laboratories, a two-vessel approach has been proposed. In this approach, coupling reaction is carried out in a round bottom flask with overhead stirrer, and the filtration (work-up) is carried out in a specially designed filtration-vessel. Using the two-vessel approach, D-Met-enkephalins (pentapeptides, Tyr-Gly-Gly-Phe-D-Met) and some of its derivatives involving lengthy reactions have been synthesized on Merrifield resin. This approach is very convenient for studying variations in reaction conditions and is good for synthesizing short peptides (<10 amino acids). By this approach 1-100 g resin can be handled in a laboratory without any additional cost. This approach can be extrapolated to solid phase syntheses as well.
Page(s): 1143-1147
ISSN: 0376-4699
Source:IJC-B Vol.46B(07) [July 2007]

Files in This Item:

File Description SizeFormat
IJCB 46B(7) (2007) 1143-1147.pdf140.2 kBAdobe PDFView/Open
 Current Page Visits: 671 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 558591 since 06-Feb-2009  Last updated on 30-Jul-2014Webmaster: nopr@niscair.res.in