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Title: Solid phase synthesis of nuclear localization signal on a new PS-TTEGDA polymer support
Authors: Samuel, Daisy
Renil, M
Pillai, V N Rajasekharan
Keywords: PS-TTEGDA polymer support;solid phase synthesis;nuclear localization signal
Issue Date: Jul-2006
Publisher: CSIR
IPC Code: Int Cl.8 C 07C
Abstract: Tetraethyleneglycol diacrylate (2%) cross-linked polystyrene a highly solvating copolymer is used for the synthesis of the peptide. The peptide is grown from the hydroxymethyl functional site introduced into the resin. The peptide H-Ala-Ala-Ala-Lys-Lys-Asn-Ser-Leu-Ala-Leu-Ser-Leu-Thr-Ala-Asp-Gln-Met-Val-Ser-Ala-Leu-Leu is the sequence of the nuclear localization signal 1 and also the hormone binding domain of the human estrogen receptor (amino acids 302-320) with polyalanine at N-terminus. The C-terminal amino acid Fmoc-Leu is anchored using DCC and HOBt where the reagents are reacted in 4:4:3 ratio. The remaining amino acids are incorporated into this support following the standard solid-phase methodology of peptide synthesis. The completely deprotected peptide is cleaved from the resin using trifluoroacetic acid in 7h, isolated in solid form and purity is checked by HPLC. The peptide is characterised by amino acid analysis and MALDI TOF MAS. Biological studies reveal that the peptide is successful in binding three proteins of 73, 55 and 28 kiloDaltons from goat uterine cytosol.
Page(s): 1740-1746
ISSN: 0975-0983(Online); 0376-4699(Print)
Appears in Collections:IJC-B Vol.45B(07) [July 2006]

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