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Title: Fishmeal extract bile salt lactose agar–A differential medium for enteric bacteria
Authors: Subbannayya, K
Udayalaxmi, J
Anugraha, M
Keywords: Bile salt lactose agar
Differential medium
Enteric bacteria
Fishmeal extract
Issue Date: Aug-2006
Publisher: CSIR
Abstract: Fishmeal extract bile salt lactose agar (FEBLA), a new differential medium for enteric bacteria was developed and evaluated for its ability to grow and differentiate lactose fermenters (LF) from non-lactose fermenters (NLF) in comparison with MacConkeys agar. Performance of FEBLA was at par with the latter. On FEBLA medium, the contrast between LF and NLF colonies was pronounced and <i style="">Klebsiella pneumoniae</i> produced more mucoid colonies than on MacConkeys agar (Hi Media). Unlike MacConkeys agar, a 24 h culture of <i style="">K. pneumoniae </i>cells on FEBLA were longer and thicker with abundant capsular material around the bacilli. <i style="">Escherichia coli</i> produced long and thick cells but only after 48h. No change in cell morphology was evident with regard to <i style="">Salmonella typhi, S. paratyphi</i> A, <i style="">Shigella flexneri</i>, <i style="">Pseudomonas aeruginosa</i>, <i style="">Proteus mirabilis</i>, <i style="">Proteus vulgaris</i>, <i style="">Citrobacter koseri</i> and <i style="">Acinetobacter baumannii</i>. Performance of the medium was controlled using <i style="">E. coli</i> and S. <i style="">flexneri</i>. FEBLA is simple, cost effective and may be a suitable alternative in the preliminary identification of enteric bacteria.<b style=""> </b>
Description: 675-678
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(08) [August 2006]

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