|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(08) [August 2006] >
| Title: | Partial purification and cytotoxic effects of Salmonella proteinous moieties on chick embryo fibroblasts |
| Authors: | Sharma, Anupma
Sharma, V D
Umapati, V
Lakhchaura, B D |
| Keywords: | Chicken embryo fibroblast
Cytotoxic
Proteins
Salmonella |
| Issue Date: | Aug-2006 |
| Publisher: | CSIR |
| Abstract: |
Salmonella enterica
serovars, viz., S. Weltevreden, S. Typhimurium, S. Gallinarum and S. Bareilly were treated with cephotaxime to release
of intracellular proteins. The cephotaxime extract (CE) was salt precipitated
with ammonium sulphate (45-70%) and dialyzed, and denoted as precipitated
dialyzed proteins (PDP). Further, both CE and PDP of Salmonella Weltevreden and PDP of rest of the serovars were
subjected to gel filtration using Sephacryl S—200HR. Different fractions along
with CE and PDP were studied for their cytotoxicity using chicken embryo
fibroblast (CEF). All the CE and PDP exerted cytotoxic effects, characterized
by rounding, detachment, shrinkage and clumping of cells with syncytia
formation. Also, the fractions eluted in the 2nd and 3rd
peaks through Sephacryl S-200HR column invariably had cytotoxic activity. It
was concluded that in place of Vero cell line, CEF cells could also be used to
test cytotoxicity.
|
| Page(s): | 666-670 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(08) [August 2006]
|
|
