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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(07) [July 2006] >
| Title: | Excretory-secretory product of Fasciola hepatica worm protects against Schistosoma mansoni infection in mice |
| Authors: | Hamed, Manal A |
| Keywords: | Antioxidant
Enzymes
Excrtory- secretory antigen
Fasciola hepatica
Schistosoma mansoni |
| Issue Date: | Jul-2006 |
| Publisher: | CSIR |
| Abstract: | The objective of this study was to evaluate
the protective immunity of excretory-secretory products of Fasciola hepatica (FhES) worm against S.mansoni infection in mice. Evaluation of FhES antigen was through
measuring worm burden, ova count, granuloma size and frequency as well as the
histopathological picture of the liver. The study was extended to determine the
level of free radical scavengers; lipid peroxide, glutathione (GSH), vitamin C,
vitamin E, catalase and superoxide dismutase (SOD). Liver function enzymes such
as aspartate aminotransferase (AST), alanine aminotransferase (ALT) and
alkaline phosphatase (ALP) were also taken into consideration. Four groups of
eight mice each were selected for this study. Group 1 served as control group.
Group 2: normal healthy mice vaccinated with FhES product. Group 3: S.mansoni infected mice for 2 months and
group 4: infected mice pre-vaccinated with FhES antigen. Vaccination schedule
comprised of a single subcutaneous injection of FhES antigen emulsified with
Freund’s complete adjuvant in a dose 0.5 mg protein/mouse, followed by
intraperitoneal injections of the same antigen without adjuvant in 3 doses/week
for 3 successive weeks. The total antigen inoculation was 5 mg protein/mouse.
The present results revealed a drastic change in all the measured parameters
after S.mansoni infection and a
noticeable improved level after vaccination with FhES antigen. It can be
concluded that FhES antigen succeeded to protect mice against schistosomiasis
by a significant reduction in worm burden, ova count, granuloma size and
number, improvement in the histopathological architecture of the liver as well
as amelioration in the antioxidant levels under investigation. |
| Page(s): | 554-561 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(07) [July 2006]
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