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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.47 [2009] >
IJEB Vol.47(11) [November 2009] >
| Title: | Solar and artificial ultraviolet-B induced erythrocytes hemolysis with photosensitizers |
| Authors: | Kumar, Sunil
Devi, Shoma
Misra, Prashasti
Priyanka |
| Keywords: | Chloroquine
Ozone depletion
Photohemolysis
Riboflavin
Ultraviolet-B |
| Issue Date: | Nov-2009 |
| Publisher: | CSIR |
| Abstract: | Aim of this study was to monitor the solar
ultraviolet-B intensity and to compare the phototoxic effect of different
intensity of natural and artificial ultraviolet-B on human red blood cells in
presence of compounds as riboflavin and chloroquine. Photohemolysis of
erythrocytes was studied under natural solar radiation and artificial
ultraviolet-B radiation of 312 nm. Monitoring of solar ultraviolet-B radiation
was performed in Garhwal region of Uttarakhand, India. Level of solar
ultraviolet-B measured show seasonal and altitudinal variations. Monthly
average of solar UV-B intensity was minimum in the month of December and
January (0.299 mw/cm2) and maximum in the month of July and August
(1.027 mw/cm2). Natural solar radiation intensities 0.402 mw/cm2
and 0.824 mw/cm2 of the month of January and June were used in the
photohemolysis experiment. Two intensities of artificial UV-B i.e. 0.824 mw/cm2
and a double intensity 1.65 mw/cm2 were also used. Results on human
erythrocytes hemolysis indicate that haemolysis was highest i.e. 71% in
chloroquine + artificial ultraviolet-B intensity (1.65 mw/cm2)
followed by 62% in chloroquine + artificial ultraviolet-B (0.824 mw/cm2)
exposed groups and 54% in natural solar radiation intensity 0.824 mw/cm2
+ chloroquine. Natural solar UV-B alone caused 17% hemolysis and show dose
response relationship. A difference in
phototoxicity was observed in natural solar and artificial UV-B of same
intensity. Artificial UV-B was found more toxic. Riboflavin was more phototoxic
in presence of solar light, while chloroquine was more phototoxic with
artificial UV-B. |
| Page(s): | 906-910 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.47(11) [November 2009]
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