NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.47 [2009] >
IJEB Vol.47(11) [November 2009] >


Title: Protocol for improved extraction and PCR amplification of genomic DNA from liverwort, Plagiochasma appendiculatum
Authors: Soni, Arvind
Kumar, Anil
Keywords: CTAB
Genomic DNA
PCR
Plagiochasma appendiculatum
Issue Date: Nov-2009
Publisher: CSIR
Abstract: A simplest method was followed for isolation of high quality genomic DNA form thallus of Plagiochasma appendiculatum that contained large quantities of polyphenols, terpenoids, tannins, contamination of high amount of RNA and polysaccharides. The method involved a modification of CTAB procedure using PVP (1%) and LiCl (4M) solution to remove polyphenols and RNA and some other binding proteins. The present protocol was found suitable for restriction enzyme digestion and random amplified polymorphic DNA (RAPD) analysis.
Page(s): 921-924
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.47(11) [November 2009]

Files in This Item:

File Description SizeFormat
IJEB 47(11) 921-924.pdf226.49 kBAdobe PDFView/Open
 Current Page Visits: 561 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 606980 since 06-Feb-2009  Last updated on 31-Oct-2014Webmaster: nopr@niscair.res.in