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IJEB Vol.47(11) [November 2009] >

Title: Protocol for improved extraction and PCR amplification of genomic DNA from liverwort, Plagiochasma appendiculatum
Authors: Soni, Arvind
Kumar, Anil
Keywords: CTAB
Genomic DNA
Plagiochasma appendiculatum
Issue Date: Nov-2009
Publisher: CSIR
Abstract: A simplest method was followed for isolation of high quality genomic DNA form thallus of Plagiochasma appendiculatum that contained large quantities of polyphenols, terpenoids, tannins, contamination of high amount of RNA and polysaccharides. The method involved a modification of CTAB procedure using PVP (1%) and LiCl (4M) solution to remove polyphenols and RNA and some other binding proteins. The present protocol was found suitable for restriction enzyme digestion and random amplified polymorphic DNA (RAPD) analysis.
Page(s): 921-924
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.47(11) [November 2009]

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