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Title: Protocol for improved extraction and PCR amplification of genomic DNA from liverwort, <i style="">Plagiochasma appendiculatum</i>
Authors: Soni, Arvind
Kumar, Anil
Keywords: CTAB
Genomic DNA
<i style="">Plagiochasma appendiculatum</i>
Issue Date: Nov-2009
Publisher: CSIR
Abstract: A simplest method was followed for isolation of high quality genomic DNA form thallus of <i style="">Plagiochasma appendiculatum</i> that contained large quantities of polyphenols, terpenoids, tannins, contamination of high amount of RNA and polysaccharides. The method involved a modification of CTAB procedure using PVP (1%) and LiCl (4<i style="">M</i>) solution to remove polyphenols and RNA and some other binding proteins. The present protocol was found suitable for restriction enzyme digestion and random amplified polymorphic DNA (RAPD) analysis.
Description: 921-924
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.47(11) [November 2009]

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