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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.47 [2009] >
IJEB Vol.47(11) [November 2009] >
| Title: | Protocol for improved extraction and PCR amplification of genomic DNA from liverwort, Plagiochasma appendiculatum |
| Authors: | Soni, Arvind
Kumar, Anil |
| Keywords: | CTAB
Genomic DNA
PCR
Plagiochasma appendiculatum |
| Issue Date: | Nov-2009 |
| Publisher: | CSIR |
| Abstract: | A simplest method was followed for isolation of high quality genomic DNA form thallus of Plagiochasma appendiculatum that contained large quantities of polyphenols, terpenoids, tannins, contamination of high amount of RNA and polysaccharides. The method involved a modification of CTAB procedure using PVP (1%) and LiCl (4M) solution to remove polyphenols and RNA and some other binding proteins. The present protocol was found suitable for restriction enzyme digestion and random amplified polymorphic DNA (RAPD) analysis. |
| Page(s): | 921-924 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.47(11) [November 2009]
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