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IJEB Vol.47(11) [November 2009] >


Title: Evaluation of aroA deletion mutant of Salmonella enterica subspecies enterica serovar Abortusequi for its vaccine candidate potential
Authors: Alam, Javed
Singh, B R
Hansda, D
Singh, V P
Verma, J C
Keywords: Abortion
Guinea pig
Mutant aroA
Salmonella enterica Abortusequi
Vaccine
Issue Date: Nov-2009
Publisher: CSIR
Abstract: The present study on a defined deletion aroA mutant (B-26) of Salmonella enterica subspecies enterica serovar Abortusequi (S. Abortusequi) for residual virulence and safety in experimental model revealed that the virulence of the strain was at no difference in any of the cell assays (caprine alveolar macrophages, bovine alveolar macrophages, guinea pig blood mononuclear cells and horse blood mononuclear cells) than that of its parent virulence plasmid cured (S-787) and wild type (E-156) strains. The mutant did not cause any apparent illness in baby guinea pigs (15 days old), adult male and female guinea pigs and also not in pregnant (54-55 days of gestation) guinea pigs through oral (4.2 × 109 cfu/ animal) and intramuscular (im) routes (4.2 × 107 cfu/ animal). In pregnant females the mutant also induced abortion as its parent (E-156) though to lesser extent (33%) than the parent strain (100%) on inoculation through intravaginal (4.2 × 109 cfu/ animal) and intraperitoneal (4.2 × 107 cfu/ animal) routes. The babies born from mutant inoculated mothers survived better and were also resistant to intraperitoneal lethal challenge (7.82 × 109 cfu/ animal) with 100% protection. Female guinea pigs challenged after 135-165 days of inoculation with the mutant afforded 100% protection from abortion and mortality caused by lethal infection (7.82 × 109 cfu/ animal) of wild type S. enterica Abortusequi (E-156). The study revealed that aroA mutant (B-26) was safe through oral and im routes for immunization and afforded 100% protection against salmonellosis for more than 5.5 months in guinea pigs. Although immunization with aroA mutant in experimental model afforded good protection against abortion and mortality induced by S. Abortusequi, further studies are needed in horses to exploit the strain’s vaccine potential in the natural host.
Page(s): 871-879
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.47(11) [November 2009]

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