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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(06) [June 2006] >
| Title: | Histological evidences of reparative and regenerative effects of ß-adrenoceptor agonists, clenbuterol and isoproterenol, in denervated rat skeletal muscle |
| Authors: | Katoch, Surender S
Garg, Asha
Sharma, Sushma |
| Keywords: | Butoxamine
Clenbuterol
Denervation
Isoproterenol hydrochloride
Nuclei
Rat gastrocnemius |
| Issue Date: | Jun-2006 |
| Publisher: | CSIR |
| Abstract: | The aim of this study was to
determine the contribution of ß-adrenoceptor
activation in the reconstruction of the structural and functional organization
of denervated skeletal muscle. ß-agonists, clenbuterol (1.2 mg/kg body weight)
and isoproterenol (2 mg/kg body weight), administration (daily oral
administration; maximum 7 days) to normal innervated rats as well as denervated
animals caused muscle hypertrophy. An increase in mean fiber diameter confirmed
this stimulated growth both in normal innervated and denervated rat
gastrocnemius muscle. Examination of muscle nuclei from treated but normal
innervated rat gastrocnemius exhibited features like large size, active nucleoplasm
and an increase in their number per fiber cross section and per mm mean fiber
length indicating towards an elevated biosynthetic activity in tissue in the
presence of ß adrenoceptor agonists. Administration of drugs to normal
innervated animals resulted in an emergence of central muscle nuclei. The
hyperactive and enlarged muscle nuclei ultimately organized themselves into
unusually elongated nuclear streaks. ß agonist treatment to denervated rats
resulted in amelioration of atrophic state of tissue characterized by
hypertrophy of muscle fibers thus lending to a restoration of structural
organization of tissue. Bizarre shapes of nuclei in denervated muscle tend to
recover to that characteristic to normal innervated muscle in presence of
clenbuterol and isoproterenol hydrochloride. All observations were confirmed by
administering butoxamine, a ß-adrenoceptor antagonist along with ß-agonists.
The results suggests that both clenbuterol and isoproterenol hydrochloride are
capable of mimicking normal innervation functions in skeletal muscle and thus
play important role in the structural and functional reorganization of tissue.
Amelioration of denervation atrophy in rat gastrocnemius in the presence of
ß-agonists supports this. |
| Page(s): | 448-458 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(06) [June 2006]
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