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Title: Reduction of vitrification in in vitro raised shoots of Chlorophytum borivilianum Sant. & Fernand., a rare potent medicinal herb
Authors: Sharma, Urvashi
Mohan, J S S
Keywords: Aphrodisiac
Growth retardant
Micropropagation
Safed musli
Shoot multiplication
Issue Date: Jun-2006
Publisher: CSIR
Abstract: Reduction of vitrification in in vitro raised shoots derived from shoot bases and immature floral buds along with inflorescence axis used as explants of C. borivilianum, a rare medicinal herb is described. Shoot multiplication was obtained on MS medium with 2 mg 1-1 benzylaminopurine (BAP) + 0.1 mg 1-1 indole-3-butyric acid (IBA) and MS medium with 2 mg 1-1 kinetin (Kin) + 0.1 mg 1-1 2,4-dichlorophenoxy acetic acid (2,4-D) from shoot bases and inflorescence axis respectively. Best multiplication rates were obtained from both the explants on MS medium with 2 mg 1-1 BAP. Vitrification of shoots in cultures appeared during the multiplication stage. Culture bottles with aerated caps reduced the vitrification to 80%. Reduction of BAP concentration from 2 mg 1-1 to zero during subsequent subcultures also minimized vitrification. Use of 0.5-2 mg 1-1 Kin produced healthy shoots when compared to BAP. In vitro raised shoots rooted on Knop salts containing iron and vitamins of MS medium, 2 mg 1-1 IBA and 0.1% activated charcoal. About 80% plantlets survived upon soil transfer. Scanning electron microscopic and image analyzer studies reveal the morphological structural differences between the leaves of normal and vitrified plantlets.
Page(s): 499-505
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(06) [June 2006]

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