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IJEB Vol.44(06) [June 2006] >
|Title: ||Reduction of vitrification in in vitro raised shoots of Chlorophytum borivilianum Sant. & Fernand., a rare potent medicinal herb|
|Authors: ||Sharma, Urvashi|
Mohan, J S S
|Issue Date: ||Jun-2006 |
|Abstract: ||Reduction of
vitrification in in vitro raised
shoots derived from shoot bases and immature floral buds along with
inflorescence axis used as explants of C.
borivilianum, a rare medicinal herb is described. Shoot multiplication was
obtained on MS medium with 2 mg 1-1 benzylaminopurine (BAP) + 0.1 mg
1-1 indole-3-butyric acid (IBA) and MS medium with 2 mg 1-1
kinetin (Kin) + 0.1 mg 1-1 2,4-dichlorophenoxy acetic acid (2,4-D)
from shoot bases and inflorescence axis respectively. Best multiplication rates
were obtained from both the explants on MS medium with 2 mg 1-1 BAP.
Vitrification of shoots in cultures appeared during the multiplication stage.
Culture bottles with aerated caps reduced the vitrification to 80%. Reduction
of BAP concentration from 2 mg 1-1 to zero during subsequent
subcultures also minimized vitrification. Use of 0.5-2 mg 1-1 Kin
produced healthy shoots when compared to BAP. In vitro raised shoots rooted on Knop salts containing iron and
vitamins of MS medium, 2 mg 1-1 IBA and 0.1% activated charcoal.
About 80% plantlets survived upon soil transfer. Scanning electron microscopic
and image analyzer studies reveal the morphological structural differences
between the leaves of normal and vitrified plantlets.|
|ISSN: ||0975-1009 (Online); 0019-5189 (Print)|
|Source:||IJEB Vol.44(06) [June 2006]|