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|Title:||Reduction of vitrification in <i style="">in vitro</i> raised shoots of <i style="">Chlorophytum borivilianum</i> Sant. & Fernand., a rare potent medicinal herb|
Mohan, J S S
|Abstract:||Reduction of vitrification in <i style="">in vitro </i>raised shoots derived from shoot bases and immature floral buds along with inflorescence axis used as explants of <i style="">C. borivilianum</i>, a rare medicinal herb is described. Shoot multiplication was obtained on MS medium with 2 mg 1<sup>-1</sup> benzylaminopurine (BAP) + 0.1 mg 1<sup>-1</sup> indole-3-butyric acid (IBA) and MS medium with 2 mg 1<sup>-1</sup> kinetin (Kin) + 0.1 mg 1<sup>-1 </sup>2,4-dichlorophenoxy acetic acid (2,4-D) from shoot bases and inflorescence axis respectively. Best multiplication rates were obtained from both the explants on MS medium with 2 mg 1<sup>-1</sup> BAP. Vitrification of shoots in cultures appeared during the multiplication stage. Culture bottles with aerated caps reduced the vitrification to 80%. Reduction of BAP concentration from 2 mg 1<sup>-1</sup> to zero during subsequent subcultures also minimized vitrification. Use of 0.5-2 mg 1<sup>-1</sup> Kin produced healthy shoots when compared to BAP. <i style="">In vitro</i> raised shoots rooted on Knop salts containing iron and vitamins of MS medium, 2 mg 1<sup>-1</sup> IBA and 0.1% activated charcoal. About 80% plantlets survived upon soil transfer. Scanning electron microscopic and image analyzer studies reveal the morphological structural differences between the leaves of normal and vitrified plantlets.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.44(06) [June 2006]|
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