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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(06) [June 2006] >
| Title: | Alkaline lipase production by Citrobacter freundii IIT-BT L139 |
| Authors: | Gunasekaran, Vijay
Kotay, Shireen Meher
Das, Debabrata |
| Keywords: | Citrobacter freundii
Enzyme production
Fermenter studies
Lipase
Optimization
Rheology |
| Issue Date: | Jun-2006 |
| Publisher: | CSIR |
| Abstract: | Around 150 lipase producing bacterial isolates were screened
from the local soils enriched with oil. Citrobacter freundii IIT-BT
L139, an isolated microbial strain, produced lipase that had high activity (8.8
U/ml) at pH 9.0 and 40oC. The 16S rDNA phylogenetic studies
showed that Citrobacter freundii belongs to the family
Enterobacteriaceae and later confirmed by the microbial identification.
Suitable C and N sources for lipase production were deduced to be starch and
peptone-urea, respectively. In a controlled fermenter (1 L), the lipase
activity was found to increase by 36% (12 Uml-1). The variation of
lipase activity, pH and dissolved oxygen (DO) during growth of the
organism in the controlled batch fermenter were monitored. The rheological
characteristics of the fermentation broth indicated that it behaved like a
Newtonian fluid throughout the fermentation. The fermentation time was
comparatively short (60 h). The lipase was also found to be substantially
resistant to common detergents. This lipase was, thus, characterized as
alkaline, thermostable and solvent stable, which was essentially desirable in
pharmaceutical, detergent and other industrial applications or production. |
| Page(s): | 485-491 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(06) [June 2006]
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