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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(05) [May 2006] >
| Title: | Secretory factors of human neuroblastoma (IMR-32) and human glioblastoma (U87MG) cell lines induce neurite outgrowths in PC12 cells |
| Authors: | Satpute, Ravindra M
Kashyap, Rajpal S
Kainthla, Rani Poonam
Purohit, Hemant J
Taori, Girdhar M
Daginawala, Hatim F |
| Keywords: | Conditioned medium
Neurites
Pheochromocytoma |
| Issue Date: | May-2006 |
| Publisher: | CSIR |
| Abstract: | Neurite outgrowth is
essential for the communication of the nervous system. The rat Pheochromocytoma
(PC12) cells are commonly used in the neuronal cell study. It is well known
that exogenous stimuli such as Nerve Growth Factor (NGF) induce neurite
outgrowth. In the present study it has been investigated whether or not the
conditioned medium from human neuroblastoma cell line (IMR-32) and human
glioblastoma cell line (U87MG) may augment neurite outgrowth in PC12 cells.
PC12 were cultured with and without conditioned media of IMR-32 and U87MG. The
result showed that both the conditioned media induce neurite outgrowth within
48 hr and stops further proliferation of PC12 cells. However no outgrowth was
noted in PC12 cells incubated without conditioned medium. In conclusion, it is
shown that both the conditioned media (IMR-32 and U87MG) have the potential to
induce the neurite outgrowth in the PC12 cells. |
| Page(s): | 367-370 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(05) [May 2006]
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