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http://nopr.niscair.res.in/handle/123456789/6496
Title: | Development of biosensor based on immobilized L-glutamate oxidase for determination of monosodium glutamate in food |
Authors: | Basu, Anjan Kumar Chattopadhyay, Parimal Roychudhuri, Utpal Chakraborty, Runu |
Keywords: | Biosensor;Food sample analysis;L-glutamate oxidase;Monosodium glutamate |
Issue Date: | May-2006 |
Publisher: | CSIR |
Abstract: | A monosodium
glutamate (MSG) biosensor with immobilized L-glutamate oxidase (L-GLOD) has
been developed and studied for analysis of MSG in sauces, soup etc. The
immobilized enzymatic membrane was attached with oxygen electrode with a push
cap system. The detection limit of the sensor was 1 mg/dl and the standard
curve was found to be linear upto 20 mg/dl. Response time of the sensor was 2
min. Cross-linking with glutaraldehyde in presence of Bovine Serum Albumin
(BSA) as a spacer molecule has been used for immobilization. Optimization of
the sensor was done with an increase in L-GLOD concentration (6.3-31.5 IU) and
also with increase in loading volume of enzyme solution (5-20 ![]() |
Page(s): | 392-398 |
URI: | http://hdl.handle.net/123456789/6496 |
ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
Appears in Collections: | IJEB Vol.44(05) [May 2006] |
Files in This Item:
File | Description | Size | Format | |
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IJEB 44(5) 392-398.pdf | 111.81 kB | Adobe PDF | View/Open |
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