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Title: Development of biosensor based on immobilized L-glutamate oxidase for determination of monosodium glutamate in food
Authors: Basu, Anjan Kumar
Chattopadhyay, Parimal
Roychudhuri, Utpal
Chakraborty, Runu
Keywords: Biosensor;Food sample analysis;L-glutamate oxidase;Monosodium glutamate
Issue Date: May-2006
Publisher: CSIR
Abstract: A monosodium glutamate (MSG) biosensor with immobilized L-glutamate oxidase (L-GLOD) has been developed and studied for analysis of MSG in sauces, soup etc. The immobilized enzymatic membrane was attached with oxygen electrode with a push cap system. The detection limit of the sensor was 1 mg/dl and the standard curve was found to be linear upto 20 mg/dl. Response time of the sensor was 2 min. Cross-linking with glutaraldehyde in presence of Bovine Serum Albumin (BSA) as a spacer molecule has been used for immobilization. Optimization of the sensor was done with an increase in L-GLOD concentration (6.3-31.5 IU) and also with increase in loading volume of enzyme solution (5-20 l). Optimization of pH and temperature was also studied. The permeability of O2 through different membrane was studied with and without immobilized L-GLOD. The enzymatic membrane was used for over 20 measurements and stability of the membrane was observed.
Page(s): 392-398
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(05) [May 2006]

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