NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(05) [May 2006] >


Title: Development of biosensor based on immobilized L-glutamate oxidase for determination of monosodium glutamate in food
Authors: Basu, Anjan Kumar
Chattopadhyay, Parimal
Roychudhuri, Utpal
Chakraborty, Runu
Keywords: Biosensor
Food sample analysis
L-glutamate oxidase
Monosodium glutamate
Issue Date: May-2006
Publisher: CSIR
Abstract: A monosodium glutamate (MSG) biosensor with immobilized L-glutamate oxidase (L-GLOD) has been developed and studied for analysis of MSG in sauces, soup etc. The immobilized enzymatic membrane was attached with oxygen electrode with a push cap system. The detection limit of the sensor was 1 mg/dl and the standard curve was found to be linear upto 20 mg/dl. Response time of the sensor was 2 min. Cross-linking with glutaraldehyde in presence of Bovine Serum Albumin (BSA) as a spacer molecule has been used for immobilization. Optimization of the sensor was done with an increase in L-GLOD concentration (6.3-31.5 IU) and also with increase in loading volume of enzyme solution (5-20 l). Optimization of pH and temperature was also studied. The permeability of O2 through different membrane was studied with and without immobilized L-GLOD. The enzymatic membrane was used for over 20 measurements and stability of the membrane was observed.
Page(s): 392-398
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(05) [May 2006]

Files in This Item:

File Description SizeFormat
IJEB 44(5) 392-398.pdf111.81 kBAdobe PDFView/Open
 Current Page Visits: 1444 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 621352 since 06-Feb-2009  Last updated on 13-Nov-2014Webmaster: nopr@niscair.res.in