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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(05) [May 2006] >
| Title: | Development of biosensor based on immobilized L-glutamate oxidase for determination of monosodium glutamate in food |
| Authors: | Basu, Anjan Kumar
Chattopadhyay, Parimal
Roychudhuri, Utpal
Chakraborty, Runu |
| Keywords: | Biosensor
Food sample analysis
L-glutamate oxidase
Monosodium glutamate |
| Issue Date: | May-2006 |
| Publisher: | CSIR |
| Abstract: | A monosodium
glutamate (MSG) biosensor with immobilized L-glutamate oxidase (L-GLOD) has
been developed and studied for analysis of MSG in sauces, soup etc. The
immobilized enzymatic membrane was attached with oxygen electrode with a push
cap system. The detection limit of the sensor was 1 mg/dl and the standard
curve was found to be linear upto 20 mg/dl. Response time of the sensor was 2
min. Cross-linking with glutaraldehyde in presence of Bovine Serum Albumin
(BSA) as a spacer molecule has been used for immobilization. Optimization of
the sensor was done with an increase in L-GLOD concentration (6.3-31.5 IU) and
also with increase in loading volume of enzyme solution (5-20  l). Optimization of pH and
temperature was also studied. The permeability of O2 through
different membrane was studied with and without immobilized L-GLOD. The
enzymatic membrane was used for over 20 measurements and stability of the
membrane was observed. |
| Page(s): | 392-398 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(05) [May 2006]
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