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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(05) [May 2006] >
| Title: | Hydrogen peroxide commences copper induced DNA damage isolated from human blood: In vitro study |
| Authors: | Prasad, Rajendra
Kumar, Sheetal R
Kumar, Sandeep |
| Keywords: | Copper
DNA damage
DNA fragmentation
Hydrogen peroxide |
| Issue Date: | May-2006 |
| Publisher: | CSIR |
| Abstract: | The present study revealed
the damaging effects of copper and hydrogen peroxide on DNA isolated from human
blood in in vitro. Ultra violet
spectral studies showed that copper and H2O2 alone (at 20
mM) caused destabilization of DNA
structure. Notwithstanding, the effect was more prominent in combination of
copper and H2O2. Further, agarose gel electrophoretic
studies revealed that neither copper nor H2O2 alone had
DNA fragmentation (up to 40 mM
concentration), while copper and H2O2 together caused
massive DNA fragmentation even at lower concentrations (4 mM copper + 4 mM H2O2).
Therefore, it was concluded from the present study that the observed
destabilization of DNA associated with alterations in configuration and
subsequently massive DNA fragmentation was in response to copper and H2O2.
Further, fluorescence spectroscopy and TUNNEL assay will address
destabilization and fragmentation of naked DNA more precisely. |
| Page(s): | 377-380 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(05) [May 2006]
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