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|Title:||Differential acetylcholinesterase activity in rat cerebrum, cerebellum and hypothalamus|
|Authors:||Roy (Pal), Rini|
Chaudhuri, Aditi Nag
|Abstract:||Acetylcholinesterase (AChE) has been purified from three different regions of rat brain using Sephadex® G 200 column. SDS PAGE (6%) showed single band for the purified AChE fractions. Purified and lyophilized AChE from different (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> precipitated fractions of three brain parts were utilized for <i style="">in vitro</i> enzyme kinetics using Dimethoate (Dmt) as inhibitor. K<sub>m</sub> values for cerebellum and hypothalamus were almost similar whereas cerebrum showed a different K<sub>m</sub> value compared to other two regions. With the drug Rivastigmine it was found that % G<sub>1</sub> and G<sub>4</sub> forms of AChE in three different parts of brain are different.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.44(05) [May 2006]|
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