Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/6491
Title: Differential acetylcholinesterase activity in rat cerebrum, cerebellum and hypothalamus
Authors: Roy (Pal), Rini
Chaudhuri, Aditi Nag
Keywords: Acetylcholinesterase activity
Cerebrum
Cerebellum
Hypothalamus
Rat
Issue Date: May-2006
Publisher: CSIR
Abstract: Acetylcholinesterase (AChE) has been purified from three different regions of rat brain using Sephadex┬« G 200 column. SDS PAGE (6%) showed single band for the purified AChE fractions. Purified and lyophilized AChE from different (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> precipitated fractions of three brain parts were utilized for <i style="">in vitro</i> enzyme kinetics using Dimethoate (Dmt) as inhibitor. K<sub>m</sub> values for cerebellum and hypothalamus were almost similar whereas cerebrum showed a different K<sub>m</sub> value compared to other two regions. With the drug Rivastigmine it was found that % G<sub>1</sub> and G<sub>4</sub> forms of AChE in three different parts of brain are different.
Description: 381-386
URI: http://hdl.handle.net/123456789/6491
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(05) [May 2006]

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