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Title: Detection of <i style="">Pasteurella multocida</i> in experimentally infected embryonated chicken eggs by PCR assay
Authors: Shivachandra, S B
Kumar, A A
Gautam, R
Joseph, S
Chaudhuri, P
Saxena, M K
Srivastava, S K
Singh, Nem
Keywords: Chick embryo
<i style="">Pasteurella multocida</i>
Issue Date: Apr-2006
Publisher: CSIR
Abstract: Applicability of polymerase chain reaction (PCR) assay to detect <i style="">Pasteurella multocida</i> in experimentally infected embryonated chicken egg was assessed in the present study. PCR assay rapidly and specifically detected the genome of <i style="">P. multocida</i> in amniotic fluid, allantoic fluid and homogenates of infected embryo and its membranes. The sensitivity of detection was as low as 20 bacterial cells/ml of allantoic or amniotic fluids. Detection of <i style="">P. multocida</i> in dead embryos by PCR was possible up to 6 and 30 days or more following storage of dead embryos at 37<sup>o</sup>C, and at 4<sup>o</sup>C as well as at -20<sup>o</sup>C, respectively. The study revealed that PCR assays could be employed directly for detection and confirmation of <i style="">P. multocida</i> infection in experimentally infected chicken embryos.
Description: 321-324
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(04) [April 2006]

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