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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(04) [April 2006] >
| Title: | Detection of Pasteurella multocida in experimentally infected embryonated chicken eggs by PCR assay |
| Authors: | Shivachandra, S B
Kumar, A A
Gautam, R
Joseph, S
Chaudhuri, P
Saxena, M K
Srivastava, S K
Singh, Nem |
| Keywords: | Chick embryo
Pasteurella multocida
PCR |
| Issue Date: | Apr-2006 |
| Publisher: | CSIR |
| Abstract: | Applicability of polymerase chain reaction
(PCR) assay to detect Pasteurella
multocida in experimentally infected embryonated chicken egg was assessed
in the present study. PCR assay rapidly and specifically detected the genome of
P. multocida in amniotic fluid,
allantoic fluid and homogenates of infected embryo and its membranes. The
sensitivity of detection was as low as 20 bacterial cells/ml of allantoic or
amniotic fluids. Detection of P. multocida
in dead embryos by PCR was possible up to 6 and 30 days or more following
storage of dead embryos at 37oC, and at 4oC as well as at
-20oC, respectively. The study revealed that PCR assays could be
employed directly for detection and confirmation of P. multocida infection in experimentally infected chicken embryos. |
| Page(s): | 321-324 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(04) [April 2006]
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