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Title: Detection of Pasteurella multocida in experimentally infected embryonated chicken eggs by PCR assay
Authors: Shivachandra, S B
Kumar, A A
Gautam, R
Joseph, S
Chaudhuri, P
Saxena, M K
Srivastava, S K
Singh, Nem
Keywords: Chick embryo;Pasteurella multocida;PCR
Issue Date: Apr-2006
Publisher: CSIR
Abstract: Applicability of polymerase chain reaction (PCR) assay to detect Pasteurella multocida in experimentally infected embryonated chicken egg was assessed in the present study. PCR assay rapidly and specifically detected the genome of P. multocida in amniotic fluid, allantoic fluid and homogenates of infected embryo and its membranes. The sensitivity of detection was as low as 20 bacterial cells/ml of allantoic or amniotic fluids. Detection of P. multocida in dead embryos by PCR was possible up to 6 and 30 days or more following storage of dead embryos at 37oC, and at 4oC as well as at -20oC, respectively. The study revealed that PCR assays could be employed directly for detection and confirmation of P. multocida infection in experimentally infected chicken embryos.
Page(s): 321-324
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(04) [April 2006]

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