|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(04) [April 2006] >
| Title: | Occurrence of non-protein low molecular weight cardiotoxin in Indian King Cobra (Ophiophagus hannah) Cantor 1836, venom |
| Authors: | Saha, Archita
Gomes, Aparna
Giri, B
Chakravarty, A K
Biswas, A K
Dasgupta, S C
Gomes, A |
| Keywords: | Cardiotoxin
Indian King Cobra
Non-protein toxin
Ophiophagus hannah
Snake venom |
| Issue Date: | Apr-2006 |
| Publisher: | CSIR |
| Abstract: | Pathophysiology due
to snakebite is a combined effect of various actions of the complex venom
constituents. Importance of protein toxins in snake envenomation is well known.
The present investigation reports the existence of non-protein/nonpetide low
molecular weight toxin in Indian King Cobra venom, which plays an important
role in envenomation consequences in experimental animal models. A group of
non-peptidic toxins (OH-NPT1) was isolated from Indian King Cobra Ophiophagus hannah by thin layer
chromatography and silica gel column chromatography. UV, IR, NMR and (ESI) TOF-MS studies
characterized the OH-NPT1 as a mixture of aliphatic acids having molecular
weights 256, 326 and 340Da. The
minimum lethal dose of OH-NPT1 was found to be 2.5  g/20g (iv) and 4g/20g (ip) in male albino mice. The cardiotoxic
property of OH-NPT1 was established through studies on isolated guinea pig
heart and auricle preparations, ECG studies in albino rat and estimation of LDH1/LDH
and CPK-MB/CPK ratio in Swiss albino mice. Commercial antiserum failed to
neutralize the lethality and cardiotoxicity of the toxin. However, calcium and
magnesium effectively neutralized the lethal action. |
| Page(s): | 279-285 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(04) [April 2006]
|
|
