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Title: Occurrence of non-protein low molecular weight cardiotoxin in Indian King Cobra (Ophiophagus hannah) Cantor 1836, venom
Authors: Saha, Archita
Gomes, Aparna
Giri, B
Chakravarty, A K
Biswas, A K
Dasgupta, S C
Gomes, A
Keywords: Cardiotoxin
Indian King Cobra
Non-protein toxin
Ophiophagus hannah
Snake venom
Issue Date: Apr-2006
Publisher: CSIR
Abstract: Pathophysiology due to snakebite is a combined effect of various actions of the complex venom constituents. Importance of protein toxins in snake envenomation is well known. The present investigation reports the existence of non-protein/nonpetide low molecular weight toxin in Indian King Cobra venom, which plays an important role in envenomation consequences in experimental animal models. A group of non-peptidic toxins (OH-NPT1) was isolated from Indian King Cobra Ophiophagus hannah by thin layer chromatography and silica gel column chromatography. UV, IR, NMR and (ESI) TOF-MS studies characterized the OH-NPT1 as a mixture of aliphatic acids having molecular weights 256, 326 and 340Da. The minimum lethal dose of OH-NPT1 was found to be 2.5 g/20g (iv) and 4g/20g (ip) in male albino mice. The cardiotoxic property of OH-NPT1 was established through studies on isolated guinea pig heart and auricle preparations, ECG studies in albino rat and estimation of LDH1/LDH and CPK-MB/CPK ratio in Swiss albino mice. Commercial antiserum failed to neutralize the lethality and cardiotoxicity of the toxin. However, calcium and magnesium effectively neutralized the lethal action.
Page(s): 279-285
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(04) [April 2006]

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